Document Detail


Neoblast-enriched fraction rescues eye formation in eye-defective planarian 'menashi' Dugesia ryukyuensis.
MedLine Citation:
PMID:  18826474     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Planarians are well known for their remarkable regenerative capacity. This capacity to regenerate is thought to be due to the presence of totipotent somatic stem cells known as 'neoblasts', which have particular morphological characteristics. The totipotency of neoblasts was supported by Baguñà's experiment, which involved the introduction of donor cells into irradiated hosts. However, since Baguñà's experiment did not include the use of a phenotypic marker, the donor cells could not be traced. In the current study, a genetic mutant planarian, menashi, an eye-defective mutant that lacks the pigmented area in the eyes, was established. This planarian is excellent for tracing the fate of cells after their introduction into irradiated hosts. To investigate the differentiation potency more directly, a neoblast-rich fraction obtained from normal worms was transplanted into an X-ray-irradiated menashi strain. Planarians that survive X-ray irradiation were developed, and we observed the pigment of the area in the eyes of the regenerating planarians. This result suggests that the neoblast-rich fraction contains cells that can proliferate and differentiate. These cells can replace the cells and structures lost by X-ray irradiation and ablation, and they can also differentiate into eye pigment cells.
Authors:
Kazuya Kobayashi; Toshiyuki Hashiguchi; Taiichiro Ichikawa; Yugo Ishino; Motonori Hoshi; Midori Matsumoto
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Development, growth & differentiation     Volume:  50     ISSN:  1440-169X     ISO Abbreviation:  Dev. Growth Differ.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-10-01     Completed Date:  2009-10-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0356504     Medline TA:  Dev Growth Differ     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  689-96     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences and Informatics, Keio University, 3-14-1 Hiyoshi, Kouhoku-ku, Yokohama 223-8522, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Eye* / cytology
Eye Abnormalities / genetics
Mutation / genetics
Planarians / cytology,  genetics,  physiology*,  radiation effects
Regeneration / physiology*
Totipotent Stem Cells / physiology*,  transplantation

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