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Neoarteries grown in vivo using a tissue-engineered hyaluronan-based scaffold.
MedLine Citation:
PMID:  18385214     Owner:  NLM     Status:  MEDLINE    
Vascular tissue engineering has emerged as a promising technology for the design of an ideal, responsive, living conduit with properties similar to that of native tissue. The missing link in tissue-engineered blood vessels is elastin biosynthesis. Several biomaterials are currently used but few support elastin biosynthesis in a 3-D array. In previous studies, we demonstrated that a hyaluronan-based scaffold (HYAFF-11) grafted in the infrarenal rat aorta successfully guided the complete regeneration of a well-functioning small-diameter (2 mm) neoartery. The aim of the present study was to test the ability of HYAFF-11 biodegradable grafts to develop into neovessels of larger size (4 mm) in a porcine model, focusing on extracellular matrix (ECM) remodeling and elastin biosynthesis. HYAFF-11 tubes (diameter 4 mm, length 5 cm) were implanted in an end-to-end fashion in the common carotid artery. Grafts were analyzed for patency with a Duplex scan every 15 days. ECM components were evaluated by histological and molecular biological methods. All the animals survived the observation period without complications. Intimal hyperplasia (initiating at the anastomotic site) and graft thrombosis led to 3 cases of partial or complete occlusion, as demonstrated by histological examination. There were no signs of stenoses or aneurysms in the remaining grafts. After 5 months, the biomaterial was almost completely degraded and replaced by a neoartery segment composed of mature smooth muscle cells, collagen, and elastin fibers organized in layers and was completely covered on the luminal surface by endothelial cells (vWF(+)). Whereas in previous small animal studies, patency rates were not optimal, those obtained in the present study using hyaluronan-based grafts of larger size confirmed the ability of these constructs to guide the development of a well-functioning neoartery, with the remarkable additional attribute of facilitating the formation of organized layers of elastin fibers.
Barbara Zavan; Vincenzo Vindigni; Sandro Lepidi; Ilaria Iacopetti; Giampiero Avruscio; Giovanni Abatangelo; Roberta Cortivo
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Publication Detail:
Type:  Journal Article     Date:  2008-04-02
Journal Detail:
Title:  FASEB journal : official publication of the Federation of American Societies for Experimental Biology     Volume:  22     ISSN:  1530-6860     ISO Abbreviation:  FASEB J.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-08-04     Completed Date:  2008-09-03     Revised Date:  2012-02-15    
Medline Journal Info:
Nlm Unique ID:  8804484     Medline TA:  FASEB J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2853-61     Citation Subset:  IM    
Department of Histology, Microbiology, and Medical Biotechnologies, University of Padova, Via Giustiniani 2, 35100 Padova, Italy.
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MeSH Terms
Arterial Occlusive Diseases / etiology,  pathology
Base Sequence
Biocompatible Materials
Bioprosthesis* / adverse effects
Blood Vessel Prosthesis* / adverse effects
Blood Vessel Prosthesis Implantation
Carotid Artery, Common / metabolism,  pathology,  surgery
DNA Primers / genetics
Elastin / biosynthesis
Extracellular Matrix / metabolism
Gene Expression
Hyaluronic Acid*
Materials Testing
Sus scrofa
Tissue Engineering / methods*
Reg. No./Substance:
0/Biocompatible Materials; 0/DNA Primers; 9004-61-9/Hyaluronic Acid; 9007-58-3/Elastin

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