Document Detail


Neisseria gonorrhoeae pilin glycan contributes to CR3 activation during challenge of primary cervical epithelial cells.
MedLine Citation:
PMID:  21371235     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Expression of type IV pili by Neisseria gonorrhoeae plays a critical role in mediating adherence to human epithelial cells. Gonococcal pilin is modified with an O-linked glycan, which may be present as a di- or monosaccharide because of phase variation of select pilin glycosylation genes. It is accepted that bacterial proteins may be glycosylated; less clear is how the protein glycan may mediate virulence. Using primary, human, cervical epithelial (i.e. pex) cells, we now provide evidence to indicate that the pilin glycan mediates productive cervical infection. In this regard, pilin glycan-deficient mutant gonococci exhibited an early hyper-adhesive phenotype but were attenuated in their ability to invade pex cells. Our data further indicate that the pilin glycan was required for gonococci to bind to the I-domain region of complement receptor 3, which is naturally expressed by pex cells. Comparative, quantitative, infection assays revealed that mutant gonococci lacking the pilin glycan did not bind to the I-domain when it is in a closed, low-affinity conformation and cannot induce an active conformation to complement receptor 3 during pex cell challenge. To our knowledge, these are the first data to directly demonstrate how a protein-associated bacterial glycan may contribute to pathogenesis.
Authors:
Michael P Jennings; Freda E-C Jen; Louise F Roddam; Michael A Apicella; Jennifer L Edwards
Related Documents :
20483315 - Dynamics of bacterial swarming.
13680205 - Toward rational control of escherichia coli o157:h7 by a phage cocktail.
4572705 - Physiology of escherichia coli k-12 during conjugation: altered recipient cell function...
8078935 - Dimorphic transition in escherichia coli and salmonella typhimurium: surface-induced di...
9012385 - Trivariate flow cytometric analysis of paraffin-embedded lung cancer specimens: applica...
24258415 - Ultrastructural and histochemical studies on guard cells.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-03-04
Journal Detail:
Title:  Cellular microbiology     Volume:  13     ISSN:  1462-5822     ISO Abbreviation:  Cell. Microbiol.     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-05-16     Completed Date:  2011-08-17     Revised Date:  2014-09-11    
Medline Journal Info:
Nlm Unique ID:  100883691     Medline TA:  Cell Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  885-96     Citation Subset:  IM    
Copyright Information:
© 2011 Blackwell Publishing Ltd.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Bacterial Adhesion
Cells, Cultured
Endocytosis
Epithelial Cells / microbiology*
Female
Fimbriae Proteins / metabolism*
Glycosylation
Humans
Macrophage-1 Antigen / metabolism*
Neisseria gonorrhoeae / pathogenicity*
Polysaccharides / metabolism*
Grant Support
ID/Acronym/Agency:
1R01AI076398/AI/NIAID NIH HHS; R01 AI076398/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Macrophage-1 Antigen; 0/Polysaccharides; 147680-16-8/Fimbriae Proteins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A role for the SNARE protein syntaxin 3 in human cytomegalovirus morphogenesis.
Next Document:  Increased detection of the HIV-1 reverse transcriptase M184V mutation using mutation-specific minori...