Document Detail

Neisseria gonorrhoeae infection causes a G1 arrest in human epithelial cells.
MedLine Citation:
PMID:  17158783     Owner:  NLM     Status:  MEDLINE    
Pathogenic bacteria can modulate and interfere with human cell cycle progression. Here we study the human pathogen Neisseria gonorrhoeae and its ability to influence and affect the cell cycle in two human target cell lines. We found that bacteria adhere equally well to cells synchronized into the different cell cycle phases of G1, S, and G2, but were unable to adhere to cells in M phase or G0 phase. In addition, using Western blot and/or flow cytometry analysis we demonstrate that bacterial infection for 24 h results in decreased levels of the cell cycle regulatory proteins cyclin B1, cyclin D1, and cyclin E. Further studies in N. gonorrhoeae-infected epithelial cells involving analysis of DNA content, bromodeoxyuridine (BrdU) incorporation, quantification of late mitotic cells and analysis of nuclear phenotype provide compelling evidence that a 24 h gonococcal infection arrests the cells in early G1 phase of the cell cycle. In summary, we present data showing that MS11 P+ strain of N. gonorrhoeae can down-regulate cyclins, important modulators of the cell cycle, and result in a G1 arrest.
Allison Jones; Ann-Beth Jonsson; Helena Aro
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-12-08
Journal Detail:
Title:  FASEB journal : official publication of the Federation of American Societies for Experimental Biology     Volume:  21     ISSN:  1530-6860     ISO Abbreviation:  FASEB J.     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-02-01     Completed Date:  2007-06-04     Revised Date:  2012-02-15    
Medline Journal Info:
Nlm Unique ID:  8804484     Medline TA:  FASEB J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  345-55     Citation Subset:  IM    
Department of Medical Biochemistry and Microbiology, Uppsala Biomedical Centre, Uppsala University, S-751 23 Uppsala, Sweden.
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MeSH Terms
Bacterial Adhesion
Blotting, Western
Cell Adhesion
Cell Cycle
Cell Line, Tumor
Cell Proliferation
Cyclin B / metabolism
Cyclin B1
Cyclin D1 / metabolism
Cyclin E / metabolism
Epithelial Cells / metabolism*,  microbiology*,  pathology
Flow Cytometry
G1 Phase*
HeLa Cells
Microscopy, Fluorescence
Neisseria gonorrhoeae / cytology,  growth & development*
Reg. No./Substance:
0/CCNB1 protein, human; 0/Cyclin B; 0/Cyclin B1; 0/Cyclin E; 136601-57-5/Cyclin D1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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