Document Detail

Negligible contribution of coronary adventitial fibroblasts to neointimal formation following balloon angioplasty in swine.
MedLine Citation:
PMID:  19252097     Owner:  NLM     Status:  MEDLINE    
Adventitial fibroblasts have previously been proposed to be a major constituent of the neointima following coronary balloon angioplasty. The present study utilized the bromodeoxyuridine (BrdU) pulse-chase technique to track adventitial fibroblast migration early after balloon injury in swine. BrdU (30 mg/kg), a marker of proliferating cells, was given intravenously 1 or 2 days after balloon angioplasty. For each time point, one animal was euthanized 24 h after injection to identify the location of the proliferating cells, while a second animal was euthanized 25 days after angioplasty to determine whether the proliferating cells migrated to form the neointima. Our results demonstrate that BrdU-positive cells were located primarily in the adventitia with all three time points 24 h after balloon angioplasty. Furthermore, when BrdU was injected on day 1 or 2 only 0.65 +/- 0.17% and 1.7 +/- 0.64%, respectively, of neointimal cells were BrdU positive on day 25. In conclusion, these results demonstrate a negligible contribution of coronary adventitial fibroblasts to neointima formation following coronary balloon angioplasty, supporting the concept that the neointima is primarily of smooth muscle cell origin.
Bradley S Fleenor; Douglas K Bowles
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2009-02-27
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  296     ISSN:  0363-6135     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-05-04     Completed Date:  2009-06-19     Revised Date:  2010-09-23    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H1532-9     Citation Subset:  IM    
Department of Biomedical Sciences, University of Missouri, Columbia, MO 65211, USA.
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MeSH Terms
Angioplasty, Transluminal, Percutaneous Coronary / adverse effects*
Bromodeoxyuridine / administration & dosage
Cell Movement*
Cell Proliferation*
Connective Tissue / pathology*
Coronary Restenosis / etiology,  pathology*
Coronary Vessels / pathology*
Disease Models, Animal
Fibroblasts / pathology*
Injections, Intramuscular
Injections, Intravenous
Muscle, Smooth, Vascular / pathology
Sus scrofa
Time Factors
Tunica Intima / pathology*
Grant Support
Reg. No./Substance:

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