Document Detail

Negative regulation of early polyomavirus expression in mouse embryonal carcinoma cells.
MedLine Citation:
PMID:  3016339     Owner:  NLM     Status:  MEDLINE    
Embryonal carcinoma cells are resistant to infection by polyomavirus (Py). We showed that this block was partially removed by inhibiting protein synthesis temporarily. The block was also partially removed when Py was coinfected with simian virus 40. Cycloheximide treatment of cells infected with Py mutants able to grow on PCC4 embryonal carcinoma cells led to 3- to 10-fold increases in the production of T-antigen-positive cells. At 31 degrees C, Py T-antigen expression was enhanced when the cells were treated with cycloheximide. We suggest that a negative labile regulatory protein(s) is synthesized in PCC4 cells, preventing the initiation of early Py transcription by binding to the noncoding sequence, especially the enhancer element B and perhaps also element A, and that the Py mutants retained a binding site(s).
C Cremisi; C Babinet
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of virology     Volume:  59     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1986 Sep 
Date Detail:
Created Date:  1986-09-17     Completed Date:  1986-09-17     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  761-3     Citation Subset:  IM    
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MeSH Terms
Antigens, Viral, Tumor / genetics*
Cell Line
Cycloheximide / pharmacology
Embryonal Carcinoma Stem Cells
Enhancer Elements, Genetic
Gene Expression Regulation*
Genes, Viral
Neoplastic Stem Cells / metabolism,  microbiology*
Polyomavirus / genetics*,  immunology
Protein Biosynthesis
Simian virus 40 / physiology
Transcription, Genetic
Reg. No./Substance:
0/Antigens, Viral, Tumor; 66-81-9/Cycloheximide

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