Document Detail

Negative regulation by glucocorticoids through interference with a cAMP responsive enhancer.
MedLine Citation:
PMID:  2838908     Owner:  NLM     Status:  MEDLINE    
Although steroid hormone receptors are known to activate gene expression by binding to specific hormone-dependent enhancers, the mechanisms by which steroids inhibit the transcription of specific genes are unknown. It is shown here by gene transfer studies that the same glucocorticoid receptor that activates gene expression can negatively regulate expression of the human glycoprotein hormone alpha-subunit gene. Glucocorticoid inhibition was conferred by a 52-nucleotide region that also contains elements crucial both for adenosine 3',5'-monophosphate (cAMP) responsiveness and for placental-specific expression of this gene and was observed only under conditions in which these elements were functioning as enhancers. Purified glucocorticoid receptor was found to bind to DNA that overlap the cAMP responsive elements sites in this region. It is hypothesized that steroid receptors negatively regulate gene expression by interfering with the activity or binding of other important transcription factors.
I E Akerblom; E P Slater; M Beato; J D Baxter; P L Mellon
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  241     ISSN:  0036-8075     ISO Abbreviation:  Science     Publication Date:  1988 Jul 
Date Detail:
Created Date:  1988-08-09     Completed Date:  1988-08-09     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  350-3     Citation Subset:  IM    
Regulatory Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037.
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MeSH Terms
Cell Line
Chorionic Gonadotropin / genetics*
Cyclic AMP / physiology*
DNA-Binding Proteins / physiology
Dexamethasone / pharmacology*
Enhancer Elements, Genetic*
Gene Expression Regulation*
Receptors, Steroid / physiology*
Regulatory Sequences, Nucleic Acid*
Transcription Factors / physiology
Reg. No./Substance:
0/Chorionic Gonadotropin; 0/DNA-Binding Proteins; 0/Receptors, Steroid; 0/Transcription Factors; 50-02-2/Dexamethasone; 60-92-4/Cyclic AMP

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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