Document Detail

Negative regulation of adipogenesis from human mesenchymal stem cells by Jun N-terminal kinase.
MedLine Citation:
PMID:  15582605     Owner:  NLM     Status:  MEDLINE    
Human mesenchymal stem cells (hMSCs) are capable of differentiating into several cell types including adipocytes, osteoblasts, and chondrocytes, under appropriate culture conditions. We found that SP600125, an inhibitor of Jun N-terminal kinase (JNK), promoted adipogenesis whereas it repressed osteogenesis from hMSCs. SP600125 increased the expression of adipogenic transcription factors, CCAAT/enhancer-binding proteins alpha and beta as well as peroxisome proliferator-activated receptor gamma2, which suggested that the chemical acted on the early steps of transcriptional regulatory cascade in adipogenesis. A gene reporter assay showed that SP600125 and a dominant negative JNK promoted a transcriptional activity dependent on the cAMP-response element (CRE). Thus, JNK represses adipogenesis from hMSCs probably by, at least in part, inhibiting the transactivating function of CRE-binding protein. Another action of JNK, phosphorylation at Ser(307) of insulin receptor substrate-1, was also predicted to contribute to the repression of adipogenesis.
Sachiko Tominaga; Tomohiro Yamaguchi; Shin-Ichiro Takahashi; Fumiko Hirose; Takashi Osumi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  326     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2004-12-07     Completed Date:  2005-01-18     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  499-504     Citation Subset:  IM    
Graduate School of Life Science, Himeji Institute of Technology, University of Hyogo, 3-2-1 Koto, Kamigori, Hyogo 678-1297, Japan.
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MeSH Terms
Adipocytes / cytology*,  drug effects,  enzymology*,  metabolism
Anthracenes / pharmacology
Biological Markers / metabolism
Cell Differentiation
Cell Line
Enzyme Inhibitors / pharmacology
JNK Mitogen-Activated Protein Kinases / antagonists & inhibitors,  metabolism*
Mesenchymal Stem Cells / cytology*,  drug effects,  enzymology*,  metabolism
Osteogenesis / drug effects
Phosphoserine / metabolism
Transcriptional Activation / drug effects
Reg. No./Substance:
0/Anthracenes; 0/Biological Markers; 0/Enzyme Inhibitors; 0/anthra(1,9-cd)pyrazol-6(2H)-one; 17885-08-4/Phosphoserine; EC Mitogen-Activated Protein Kinases

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