Document Detail


Negative regulation of adipogenesis from human mesenchymal stem cells by Jun N-terminal kinase.
MedLine Citation:
PMID:  15582605     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human mesenchymal stem cells (hMSCs) are capable of differentiating into several cell types including adipocytes, osteoblasts, and chondrocytes, under appropriate culture conditions. We found that SP600125, an inhibitor of Jun N-terminal kinase (JNK), promoted adipogenesis whereas it repressed osteogenesis from hMSCs. SP600125 increased the expression of adipogenic transcription factors, CCAAT/enhancer-binding proteins alpha and beta as well as peroxisome proliferator-activated receptor gamma2, which suggested that the chemical acted on the early steps of transcriptional regulatory cascade in adipogenesis. A gene reporter assay showed that SP600125 and a dominant negative JNK promoted a transcriptional activity dependent on the cAMP-response element (CRE). Thus, JNK represses adipogenesis from hMSCs probably by, at least in part, inhibiting the transactivating function of CRE-binding protein. Another action of JNK, phosphorylation at Ser(307) of insulin receptor substrate-1, was also predicted to contribute to the repression of adipogenesis.
Authors:
Sachiko Tominaga; Tomohiro Yamaguchi; Shin-Ichiro Takahashi; Fumiko Hirose; Takashi Osumi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  326     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2004-12-07     Completed Date:  2005-01-18     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  499-504     Citation Subset:  IM    
Affiliation:
Graduate School of Life Science, Himeji Institute of Technology, University of Hyogo, 3-2-1 Koto, Kamigori, Hyogo 678-1297, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adipocytes / cytology*,  drug effects,  enzymology*,  metabolism
Anthracenes / pharmacology
Biological Markers / metabolism
Cell Differentiation
Cell Line
Enzyme Inhibitors / pharmacology
Humans
JNK Mitogen-Activated Protein Kinases / antagonists & inhibitors,  metabolism*
Mesenchymal Stem Cells / cytology*,  drug effects,  enzymology*,  metabolism
Osteogenesis / drug effects
Phosphoserine / metabolism
Transcriptional Activation / drug effects
Chemical
Reg. No./Substance:
0/Anthracenes; 0/Biological Markers; 0/Enzyme Inhibitors; 0/anthra(1,9-cd)pyrazol-6(2H)-one; 17885-08-4/Phosphoserine; EC 2.7.11.24/JNK Mitogen-Activated Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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