| Negative regulation of the RTBV promoter by designed zinc finger proteins. | |
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MedLine Citation:
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PMID: 20169401 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The symptoms of rice tungro disease are caused by infection by a DNA-containing virus, rice tungro bacilliform virus (RTBV). To reduce expression of the RTBV promoter, and to ultimately reduce virus replication, we tested three synthetic zinc finger protein transcription factors (ZF-TFs), each comprised of six finger domains, designed to bind to sequences between -58 and +50 of the promoter. Two of these ZF-TFs reduced expression from the promoter in transient assays and in transgenic Arabidopsis thaliana plants. One of the ZF-TFs had significant effects on plant regeneration, apparently as a consequence of binding to multiple sites in the A. thaliana genome. Expression from the RTBV promoter was reduced by approximately 45% in transient assays and was reduced by up to 80% in transgenic plants. Co-expression of two different ZF-TFs did not further reduce expression of the promoter. These experiments suggest that ZF-TFs may be used to reduce replication of RTBV and thereby offer a potential method for control of an important crop disease. |
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Authors:
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M Isabel Ordiz; Laurent Magnenat; Carlos F Barbas; Roger N Beachy |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-02-19 |
Journal Detail:
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Title: Plant molecular biology Volume: 72 ISSN: 1573-5028 ISO Abbreviation: Plant Mol. Biol. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-12 Completed Date: 2010-06-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9106343 Medline TA: Plant Mol Biol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 621-30 Citation Subset: IM |
Affiliation:
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Donald Danforth Plant Science Center, 975 N. Warson Road, St. Louis, MO 63132, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Arabidopsis
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genetics,
virology Base Sequence Gene Expression Regulation, Viral Glucuronidase Molecular Sequence Data Oryza sativa / genetics, virology Plant Diseases / virology* Plants, Genetically Modified / virology Promoter Regions, Genetic* RNA, Messenger Transcription Factors / chemistry, genetics* Tungrovirus / genetics* Viral Proteins / chemistry, genetics* Virus Replication / genetics Zinc Fingers |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 0/Transcription Factors; 0/Viral Proteins; EC 3.2.1.31/Glucuronidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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