Document Detail

Negative inotropic effect of interleukin-2 in the isolated ventricular myocytes: role of NO/sGC pathway.
MedLine Citation:
PMID:  17271079     Owner:  NLM     Status:  PubMed-not-MEDLINE    
The present study is to investigate the effect and possible mechanism of interleukin-2 (IL-2) on the cell contractility in isolated rat cardiomyocytes. Ventricular myocytes were isolated from adult male Sprague-Dawley rats. Contractile responses were evaluated by use of the video tracking system. Contractile properties analyzed in cells electrically stimulated at 0.2Hz included peak velocity of cell shortening (+dL/dtmax), peak velocity of cell relengthening (-dL/dtmax), contraction amplitude (dL) and end-diastolic cell length. Calcium transients of ventricular myocytes were determined by the spectrofluorometric techniques. IL-2 (2.0, 10, 50, 200 and 1000 U/ml) exhibited a dose-dependent inhibition in +dL/dtmax, -dL/dtmax, dL and end-diastolic cell length. Pretreatment with the nitric oxide synthase inhibitor Nomega-nitro-L-arginine methyl ester (L-NAME, 100 microM) and 1H-[1,2,4]oxadiazolo[4,3a]quinoxalin-1-one (ODQ, soluble guanylyl cyclase inhibitor) blocked IL-2-induced inhibition of the contractility. IL-2 at 200 U/ml decreased the amplitude of the [Ca2+]i transient. Pretreatment with the L-NAME or ODQ abolished IL-2-induced inhibition of amplitude of the calcium transient. We conclude that the depressant effect of IL-2 on the contraction and calcium transient of isolated ventricular myocytes is mediated by nitric oxide/soluble guanylyl cyclase pathway.
H-P Wang; Q Xia; H-F Jin; C-M Cao; G-H Lin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference     Volume:  5     ISSN:  1557-170X     ISO Abbreviation:  Conf Proc IEEE Eng Med Biol Soc     Publication Date:  2004  
Date Detail:
Created Date:  2007-02-02     Completed Date:  2007-06-08     Revised Date:  2014-08-21    
Medline Journal Info:
Nlm Unique ID:  101243413     Medline TA:  Conf Proc IEEE Eng Med Biol Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3632-5     Citation Subset:  -    
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