| Negative correlation of L-glutamine concentration with proliferation rate in rat hepatomas. | |
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MedLine Citation:
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PMID: 6141511 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The concentration of L-glutamine was determined in freeze-clamped samples of normal liver of adult male fed rats (5.7-6.1 mumol/g) and in transplantable hepatomas of vastly different proliferative rates. The L-glutamine concentration in the slowly growing hepatomas was in the range of the normal liver and it decreased in relation to the increase of hepatoma growth rate, in the most rapidly growing tumors amounting to 12% of that of normal liver. In 24-hour regenerating liver, the glutamine content was slightly reduced (by 17%). In normal rat organs of high cell renewal, such as testis, intestinal mucosa, spleen, and thymus, the L-glutamine concentration was 18 to 46% of that of normal rat liver. The L-glutamine content was similar in rat brain and liver, but it was 1.6-fold higher in the heart, and low in the blood. Glutamine synthetase (EC 6.3. 1.3) activity in normal adult liver of ACI/N strain rats was 1,000 nmol per hr per mg protein; the activity increased in the very slowly growing hepatoma 20, but decreased markedly in all the other hepatomas. Thus, glutamine synthetase activity was essentially transformation-linked. The negative correlation of glutamine content with growth rate in transplanted hepatomas appears to be more closely linked with the activities of enzymes that utilize glutamine. The low L-glutamine concentration in the rapidly growing hepatomas provides a potential marker for anti-glutamine chemotherapy selectively targeted against the glutamine-utilizing enzymes. |
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Authors:
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J S Sebolt; G Weber |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Life sciences Volume: 34 ISSN: 0024-3205 ISO Abbreviation: Life Sci. Publication Date: 1984 Jan |
Date Detail:
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Created Date: 1984-02-29 Completed Date: 1984-02-29 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0375521 Medline TA: Life Sci Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 301-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Differentiation Cell Division Glutamate-Ammonia Ligase / metabolism Glutamine / metabolism* Hepatectomy Liver / cytology, enzymology, metabolism* Liver Neoplasms, Experimental / enzymology, metabolism*, pathology Liver Regeneration Male Rats Rats, Inbred ACI Rats, Inbred BUF Tissue Distribution |
| Grant Support | |
ID/Acronym/Agency:
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CA-05034/CA/NCI NIH HHS; CA-13526/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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56-85-9/Glutamine; EC 6.3.1.2/Glutamate-Ammonia Ligase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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