| Negative control of bacterial DNA replication by a cell cycle regulatory protein that binds at the chromosome origin. | |
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MedLine Citation:
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PMID: 9419339 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Caulobacter crescentus divides asymmetrically generating two distinct cell types at each cell division: a stalked cell competent for DNA replication, and a swarmer cell that is unable to initiate DNA replication until it differentiates into a stalked cell later in the cell cycle. The CtrA protein, a member of the response regulator family of the two-component signal transduction system, controls multiple cell cycle processes in Caulobacter and is present in swarmer cells but absent from stalked cells. We report that CtrA binds five sites within the chromosome replication origin in vitro. These sites overlap an essential DnaA box and a promoter in the origin that is essential for replication initiation. Analysis of mutant alleles of ctrA and point mutations in one of the CtrA binding sites in the origin demonstrate that CtrA represses replication in vivo. CtrA-mediated repression at the origin thus restricts replication to the stalked cell type. Thus, the direct coupling of chromosome replication with the cell cycle is mediated by the ubiquitous two-component signaling proteins. |
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Authors:
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K C Quon; B Yang; I J Domian; L Shapiro; G T Marczynski |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 95 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1998 Jan |
Date Detail:
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Created Date: 1998-02-18 Completed Date: 1998-02-18 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 120-5 Citation Subset: IM |
Affiliation:
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Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alleles Bacterial Proteins / genetics, metabolism* Binding Sites Caulobacter / cytology* Cell Cycle* Chromosomes, Bacterial / metabolism* DNA Footprinting DNA Replication* DNA, Bacterial / biosynthesis* DNA-Binding Proteins* Mutagenesis, Site-Directed Sequence Analysis, DNA Transcription Factors* |
| Grant Support | |
ID/Acronym/Agency:
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GM51426/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/CtrA protein, Caulobacter; 0/DNA, Bacterial; 0/DNA-Binding Proteins; 0/Transcription Factors |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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