Document Detail


Neferine increases STI571 chemosensitivity via inhibition of P-gp expression in STI571-resistant K562 cells.
MedLine Citation:
PMID:  21261505     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We investigated the effects of neferine (Nef) on STI571 sensitivity and the possible mechanism in STI571-resistant K562/G01 cells. We observed cell proliferation by the modified MTT (methyl thiazolyl tetrazolium) assay. We determined the intracellular concentration of STI571 in K562/G01 cells by high-performance liquid chromatography (HPLC), the expression of P-glycoprotein (P-gp) by Western blotting, and the expression of MDR-1 mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR). We observed that drug resistance to STI571 for K562/G01 cells was 43.99-fold higher than that for K562 cells. We also observed that a low concentration of Nef (<8 μM) and verapamil hydrochloride (VRP) (<10 μM) showed no direct cytotoxicity but significantly reduced the 50% cell growth inhibitory concentration (IC(50)) values of STI571 in K562/G01 cells. The reverse multiples for 8 μM Nef and 10 μM VRP were approximately two-fold. Both Nef (8 μM) and VRP (10 μM) decreased MDR-1 mRNA and P-gp protein expression and increased intracellular STI57I concentrations significantly in K562/G01 cells. Nef is a candidate chemical that can increase STI571 chemosensitivity in STI571-resistant K562 cells by inhibition of P-gp expression and increasing intracellular STI571 accumulation.
Authors:
Qun Qin; Xiao-Ping Chen; Zhou-Sheng Yang; Yu-Hang Xiao; Hui Min; Yuan-Jian Li
Related Documents :
15571775 - Exploring permeability of escherichia coli competence using quantum dots as fluorescent...
7042335 - Exclusive localization of colicin a in cell cytoplasm of producing bacteria.
7007325 - Lipid synthesis during the escherichia coli cell cycle.
20692935 - In vitro cytotoxicity of neutrophil-like human hl-60 cells undergoing an oxidative burs...
20143855 - Microbioreactors for raman microscopy of stromal cell differentiation.
4568725 - Translation of bacteriophage q rna by cytoplasmic extracts of mammalian cells.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-1-24
Journal Detail:
Title:  Leukemia & lymphoma     Volume:  -     ISSN:  1029-2403     ISO Abbreviation:  -     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-1-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9007422     Medline TA:  Leuk Lymphoma     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Pharmacy, Xiangya Hospital, Central South University, Changsha, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Primary mediastinal large B-cell lymphoma: optimal therapy and prognostic factors in 41 consecutive ...
Next Document:  Reduction in waiting time for diagnosis and treatment of head and neck cancer - a fast track study.