Document Detail

Nedd1 expression as a marker of dynamic centrosomal localization during mouse embryonic development.
MedLine Citation:
PMID:  18239929     Owner:  NLM     Status:  MEDLINE    
As the primary microtubule-organizing centre of the mammalian cell, the centrosome plays many important roles during cell growth and organization. This is evident across a broad range of cell types and processes, such as the proliferation, differentiation and polarity of neural cells. Additionally, given its localization and function, there are likely to be many more processes that rely on the centrosome that have not yet been characterized. Currently, little is known about centrosomal dynamics during mammalian development. In this study, we have analyzed Nedd1 protein expression to characterize the localization of the centrosome during some aspects of mouse embryogenesis. Using a Nedd1 antibody we have demonstrated the colocalization of Nedd1 with centrosomal markers. We found strong expression of Nedd1, and therefore the centrosome, in highly proliferating cells during neural development. Additionally, Nedd1 was found to have high expression in the cytoplasm of a subset of cells in the dorsal root ganglia. We have also shown a distinct, polarized centrosomal localization of Nedd1 in the developing lens, retina and other polarized tissues. This study reveals the localization of Nedd1 and the centrosome during important processes in mouse embryogenesis and provides a basis for further study into its role in development.
Jantina A Manning; Paul A Colussi; Simon A Koblar; Sharad Kumar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-01
Journal Detail:
Title:  Histochemistry and cell biology     Volume:  129     ISSN:  0948-6143     ISO Abbreviation:  Histochem. Cell Biol.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-16     Completed Date:  2008-11-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9506663     Medline TA:  Histochem Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  751-64     Citation Subset:  IM    
Hanson Institute, Institute of Medical and Veterinary Science, PO Box 14, Rundle Mall, Adelaide, SA, 5000, Australia.
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MeSH Terms
Cell Line
Cell Line, Tumor
Cell Polarity
Central Nervous System / embryology,  metabolism*
Centrosome / metabolism*
Cilia / metabolism
Embryonic Development
Eye / embryology,  metabolism*
Ganglia, Spinal / embryology,  metabolism
Microtubule-Associated Proteins / metabolism*
Organ Specificity
Reg. No./Substance:
0/Microtubule-Associated Proteins; 0/NEDD1 protein, human; 155982-08-4/Nedd1 protein, mouse

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