Document Detail


Nedd1 expression as a marker of dynamic centrosomal localization during mouse embryonic development.
MedLine Citation:
PMID:  18239929     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
As the primary microtubule-organizing centre of the mammalian cell, the centrosome plays many important roles during cell growth and organization. This is evident across a broad range of cell types and processes, such as the proliferation, differentiation and polarity of neural cells. Additionally, given its localization and function, there are likely to be many more processes that rely on the centrosome that have not yet been characterized. Currently, little is known about centrosomal dynamics during mammalian development. In this study, we have analyzed Nedd1 protein expression to characterize the localization of the centrosome during some aspects of mouse embryogenesis. Using a Nedd1 antibody we have demonstrated the colocalization of Nedd1 with centrosomal markers. We found strong expression of Nedd1, and therefore the centrosome, in highly proliferating cells during neural development. Additionally, Nedd1 was found to have high expression in the cytoplasm of a subset of cells in the dorsal root ganglia. We have also shown a distinct, polarized centrosomal localization of Nedd1 in the developing lens, retina and other polarized tissues. This study reveals the localization of Nedd1 and the centrosome during important processes in mouse embryogenesis and provides a basis for further study into its role in development.
Authors:
Jantina A Manning; Paul A Colussi; Simon A Koblar; Sharad Kumar
Related Documents :
19914879 - Entry of burkholderia organisms into respiratory epithelium: cftr, microfilament and mi...
3095609 - Evaluation of pseudomonas aeruginosa influence on the cytoskeleton of hep-2 cells.
15809029 - Spindle positioning by cortical pulling forces.
8688079 - Activation of the budding yeast spindle assembly checkpoint without mitotic spindle dis...
18508459 - The kinetochore and spindle checkpoint in mammals.
17228329 - Spindle regulation in neural precursors of flies and mammals.
7174739 - Growth and death of hela cells in the presence of caffeine.
170989 - Uptake of [3h] thymidine and cell dna synthesis during the early multiplication phase o...
3813769 - Increased efficacy of antidromic and orthodromic activation of cat alpha motoneurons up...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-01
Journal Detail:
Title:  Histochemistry and cell biology     Volume:  129     ISSN:  0948-6143     ISO Abbreviation:  Histochem. Cell Biol.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-16     Completed Date:  2008-11-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9506663     Medline TA:  Histochem Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  751-64     Citation Subset:  IM    
Affiliation:
Hanson Institute, Institute of Medical and Veterinary Science, PO Box 14, Rundle Mall, Adelaide, SA, 5000, Australia.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cell Line, Tumor
Cell Polarity
Central Nervous System / embryology,  metabolism*
Centrosome / metabolism*
Cilia / metabolism
Embryonic Development
Eye / embryology,  metabolism*
Ganglia, Spinal / embryology,  metabolism
Humans
Mice
Microtubule-Associated Proteins / metabolism*
Organ Specificity
Chemical
Reg. No./Substance:
0/Microtubule-Associated Proteins; 0/NEDD1 protein, human; 155982-08-4/Nedd1 protein, mouse

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Visual field defects in acute optic neuritis--distribution of different types of defect pattern, ass...
Next Document:  Tertiary individual prevention of occupational skin diseases: a decade's experience with recalcitran...