| Necrosis of infectious pancreatic necrosis virus (IPNV) infected cells rarely is preceded by apoptosis. | |
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MedLine Citation:
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PMID: 15763143 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Infectious pancreatic necrosis virus (IPNV)-infected cells were labeled with Annexin V and propidium iodide in order to determine the proportion of cells, which developed necrosis and/or apoptosis during the time course of infection. Contrasting with earlier reports, we found that at any time during IPNV multiplication cycle, the percentage of apoptotic cells never exceeded the 12% of the whole population of the infected cells. In addition, the percentage of necrotic cells increased continuously until reaching the 75% of the infected cells at 15 h post infection. Apoptotic cells were also identified by in situ terminal deoxynucleotidyl transferase-mediated BrdUTP nick end labeling (TUNEL). Our results are in accordance with the idea that apoptosis rarely precedes necrosis. |
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Authors:
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Juan Carlos Espinoza; Marcia Cortés-Gutierrez; Juan Kuznar |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2004-12-22 |
Journal Detail:
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Title: Virus research Volume: 109 ISSN: 0168-1702 ISO Abbreviation: Virus Res. Publication Date: 2005 May |
Date Detail:
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Created Date: 2005-03-14 Completed Date: 2005-07-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8410979 Medline TA: Virus Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 133-8 Citation Subset: IM |
Affiliation:
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Laboratorio de Bioquímica y Virología, Instituto de Ciencias Biológicas y Químicas, Facultad de Ciencias, Casilla 50 30, Universidad de Valparaíso, Valparaíso, Chile. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Annexin A5 / metabolism Apoptosis* Cell Line Cytopathogenic Effect, Viral* In Situ Nick-End Labeling Infectious pancreatic necrosis virus / physiology* Necrosis* Propidium / metabolism Salmon Staining and Labeling Virus Assembly |
| Chemical | |
Reg. No./Substance:
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0/Annexin A5; 36015-30-2/Propidium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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