Document Detail

Near-infrared reflectance spectroscopy-based methods for phytase registration in feed industry.
MedLine Citation:
PMID:  17715890     Owner:  NLM     Status:  MEDLINE    
The application of biotechnological products in the feed industry has undergone explosive growth in recent years, and phytase from microorganism accounts for one-third of the entire feed enzyme market. In this study, some differences in the composition of protein and denaturation temperature between two commercial phytases were determined by HPLC and differential scanning calorimetry, which were derived from the same origin of E. coli. At the same time, we found that it was advantageous for near-infrared reflectance spectroscopy (NIRS) to display the protein differences in the commercial phytase, which is most important for ensuring the traceability of biotechnological products in feed and food safety control. Furthermore, NIRS could track the changes in phytase during the spray-drying process and the change of enzyme activity during storage of phytase. Our experiments proved that the information from NIRS could describe well the individual characteristics of the commercial phytase, which indicated that near-infrared reflectance spectra could be exploited to use in the registration system of commercial phytase.
Haifeng Yang; Xiaowen Lv; Jing Wang; Junguo Li; Hui Li; Yuchang Qin
Publication Detail:
Type:  Journal Article     Date:  2007-08-23
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  55     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-09-13     Completed Date:  2007-12-12     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7667-75     Citation Subset:  IM    
Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
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MeSH Terms
6-Phytase / analysis*
Animal Feed / analysis*
Chromatography, High Pressure Liquid
Escherichia coli / enzymology
Hot Temperature
Protein Denaturation
Spectroscopy, Near-Infrared / methods*
Reg. No./Substance:

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