Document Detail


Near-infrared reflectance analysis to evaluate the nitrogen and fat content of human milk in neonatal intensive care units.
MedLine Citation:
PMID:  18192331     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To validate near-infrared reflectance analysis (NIRA) as a fast, reliable and suitable method for routine evaluation of human milk's nitrogen and fat content. SETTING: One neonatal intensive care unit. PATIENTS: 124 samples of expressed human milk (55 from preterm mothers and 69 from term mothers). INTERVENTION: Measurement of nitrogen and fat content by NIRA and traditional methods (Gerber method for fat and Kjeldahl method for nitrogen). MAIN OUTCOME MEASURES: Agreement between NIRA and traditional methods. Variability in fat and nitrogen content of human milk. RESULTS: A strong agreement was found between the results of traditional methods and NIRA for both fat and nitrogen content (expressed as g/100 g of milk) in term (mean fat content: NIRA = 2.76; Gerber = 2.76; mean nitrogen content: NIRA = 1.88; Kjeldahl = 1.92) and preterm (mean fat content: NIRA = 3.56; Gerber = 3.52; mean nitrogen content: NIRA = 1.91; Kjeldahl = 1.89) mothers' milk. Nitrogen content of the milk samples, measured by NIRA, ranged from 1.18 g/100 g to 2.71 g/100 g of milk in preterm milk and from 1.48 g/100 g to 2.47 g/100 g in term milk; fat content ranged from 1.27 g/100 g to 6.23 g/100 g of milk in preterm milk and from 1.01 g/100 g to 6.01 g/100 g of milk in term milk. CONCLUSION: NIRA can be used as a quick and reliable tool for routine monitoring of macronutrient content of human milk and for devising individualised human milk fortification regimens in the feeding of very premature infants.
Authors:
L Corvaglia; B Battistini; V Paoletti; A Aceti; M G Capretti; G Faldella
Publication Detail:
Type:  Journal Article     Date:  2008-01-11
Journal Detail:
Title:  Archives of disease in childhood. Fetal and neonatal edition     Volume:  93     ISSN:  1468-2052     ISO Abbreviation:  Arch. Dis. Child. Fetal Neonatal Ed.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-08-25     Completed Date:  2008-09-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9501297     Medline TA:  Arch Dis Child Fetal Neonatal Ed     Country:  England    
Other Details:
Languages:  eng     Pagination:  F372-5     Citation Subset:  AIM; IM    
Affiliation:
Istituto Clinico di Pediatria Preventiva e Neonatologia, Policlinico S.Orsola-Malpighi, Università di Bologna, Via Massarenti 11-40138 Bologna, Italy. luigi.corvaglia@unibo.it
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MeSH Terms
Descriptor/Qualifier:
Breast Feeding*
Fats / analysis*
Female
Humans
Infant Formula
Infant, Newborn
Infant, Premature / growth & development*
Intensive Care Units, Neonatal
Milk, Human / chemistry*
Mothers
Nitrogen / analysis*
Pregnancy
Spectrophotometry, Infrared*
Treatment Outcome
Chemical
Reg. No./Substance:
0/Fats; 7727-37-9/Nitrogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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