Document Detail

Near-infrared micro-Raman spectroscopy for in vitro detection of cervical cancer.
MedLine Citation:
PMID:  20223058     Owner:  NLM     Status:  MEDLINE    
Near-infrared Raman spectroscopy is a powerful analytical tool for detecting critical differences in biological samples with minimum interference in the Raman spectra from the native fluorescence of the samples. The technique is often suggested as a potential screening tool for cancer. In this article we report in vitro Raman spectra of squamous cells in normal and cancerous cervical human tissue from seven patients, which have good signal-to-noise ratio and which were found to be reproducible. These preliminary results show that several Raman features in these spectra could be used to distinguish cancerous cervical squamous cells from normal cervical squamous cells. In general, the Raman spectra of cervical cancer cells show intensity differences compared to those of normal squamous cell spectra. For example, several well-defined Raman peaks of collagen in the 775 to 975 cm(-1) region are observed in the case of normal squamous cells, but these are below the detection limit of normal Raman spectroscopy in the spectra of invasive cervical cancer cells. In the high frequency 2800 to 3100 cm(-1) region, it is found that the peak area under the CH stretching band is lower by a factor of approximately six in the spectra of cervical cancer cells as compared with that of the normal cells. The Raman chemical maps of regions of cancer and normal cells in the cervical epithelium made from the spectral features in the 775 to 975 cm(-1) and 2800 to 3100 cm(-1) regions are also found to show good correlation with each other.
Lori E Kamemoto; Anupam K Misra; Shiv K Sharma; Marc T Goodman; Hugh Luk; Ava C Dykes; Tayro Acosta
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Applied spectroscopy     Volume:  64     ISSN:  1943-3530     ISO Abbreviation:  Appl Spectrosc     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-12     Completed Date:  2010-06-17     Revised Date:  2014-09-11    
Medline Journal Info:
Nlm Unique ID:  0372406     Medline TA:  Appl Spectrosc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  255-61     Citation Subset:  IM    
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MeSH Terms
Diagnostic Imaging / methods*
Diagnostic Techniques, Obstetrical and Gynecological*
Microtechnology / methods*
Neoplasms, Squamous Cell / diagnosis*
Spectrum Analysis, Raman / methods*
Uterine Cervical Neoplasms / diagnosis*
Grant Support
N01 PC035137/PC/NCI NIH HHS; N01-PC-35137/PC/NCI NIH HHS; R01 CA058598/CA/NCI NIH HHS; R01 CA058598-12/CA/NCI NIH HHS; R01-CA-58598/CA/NCI NIH HHS

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