Document Detail


Near-infrared imaging of injured tissue in living subjects using IR-820.
MedLine Citation:
PMID:  19344575     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The unprecedented increase in preclinical studies necessitates high-throughput, inexpensive, and straightforward methods for evaluating diseased tissues. Near-infrared imaging of live subjects is a versatile, cost-effective technology that can be effectively used in a variety of pathologic conditions. We have characterized an inexpensive optoelectronic chemical, IR-820, as an infrared blood pool contrast agent to detect and quantify diseased tissue in live animals. IR-820 has maximal excitation and emission wavelengths of 710 and 820 nm, respectively. IR-820 emission is significantly improved in vivo on serum binding to albumin, and elimination occurs predominantly via the gastrointestinal tract. We demonstrate the utility of this contrast agent for serially imaging of traumatized tissue (muscle), tissue following reperfusion (eg, stroke), and tumors. IR-820 can also be employed to map regional lymph nodes. This novel contrast agent is anticipated to be a useful and an inexpensive tool for screening a wide variety of preclinical models of human diseases.
Authors:
Suresh I Prajapati; Carlo O Martinez; Ali N Bahadur; Isabel Q Wu; Wei Zheng; James D Lechleiter; Linda M McManus; Gary B Chisholm; Joel E Michalek; Paula K Shireman; Charles Keller
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Molecular imaging     Volume:  8     ISSN:  1535-3508     ISO Abbreviation:  Mol Imaging     Publication Date:    2009 Jan-Feb
Date Detail:
Created Date:  2009-04-06     Completed Date:  2009-06-08     Revised Date:  2014-09-13    
Medline Journal Info:
Nlm Unique ID:  101120118     Medline TA:  Mol Imaging     Country:  United States    
Other Details:
Languages:  eng     Pagination:  45-54     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Capillary Permeability / physiology
Contrast Media / diagnostic use*,  pharmacokinetics*
Indocyanine Green / analogs & derivatives*,  diagnostic use,  pharmacokinetics
Metabolic Clearance Rate / physiology
Mice
Mice, Hairless
Mice, Inbred C57BL
Mice, Transgenic
Models, Biological
Recovery of Function / physiology
Spectroscopy, Near-Infrared / methods*
Time Factors
Wounds and Injuries / diagnosis*,  metabolism,  rehabilitation
Grant Support
ID/Acronym/Agency:
HL074236/HL/NHLBI NIH HHS; HL090196/HL/NHLBI NIH HHS; R01 CA133229/CA/NCI NIH HHS; R01 CA133229-02/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Contrast Media; 0/IR 820; IX6J1063HV/Indocyanine Green
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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