Document Detail


Navigation to the graveyard-induction of various pathways of necrosis and their classification by flow cytometry.
MedLine Citation:
PMID:  23733565     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Apoptosis and necrosis reflect the program of cell death employed by a dying cell and the final stage of death, respectively. Whereas apoptosis is defined as a physiological, highly organized cell death process, necrosis is commonly considered to be accidental and uncontrolled. Physiological and weak pathological death stimuli preferentially induce apoptosis, while harsh non-physiological insults often immediately instigate (primary) necrosis. If an apoptosing cell transits into a phase of plasma membrane disintegration, this stage of death is referred to as secondary or post-apoptotic necrosis.Here, we present several conditions that stimulate primary and/or secondary necrosis and show that necrosis displays considerably different time courses. For subclassification of necrotic phenotypes we employed a flow cytometric single-tube 4-color staining technique including annexin A5-FITC, propidium iodide, DiIC1(5), and Hoechst 33342.
Authors:
Christina Janko; Luis Munoz; Ricardo Chaurio; Christian Maueröder; Christian Berens; Kirsten Lauber; Martin Herrmann
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  1004     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2013-06-04     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3-15     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany.
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