Document Detail


Nature and properties of a Staphylococcus epidermidis bacteriocin.
MedLine Citation:
PMID:  5079064     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Staphylococcin 1580, produced by Staphylococcus epidermidis 1580, consisted of 41.8% protein, 34% carbohydrate, and 21.9% lipid. In the protein fraction, the acidic amino acids, glutamic and aspartic acid, and the neutral amino acids, glycine and alanine, predominated. Neutral sugars consisted of glucose, galactose, and fucose in a molar ratio of 6:3:1. The purified bacteriocin was not inactivated by heating for 15 min at 120 C in the presence of 0.5% serum albumin and was stable in the pH range from 3.5 to 8.5. The compound was sensitive to the action of the proteolytic enzymes trypsin, Pronase, and chymotrypsin. All gram-negative bacteria tested were resistant; a large number of gram-positive bacteria were sensitive to staphylococcin 1580 action. Growth of stable staphylococcal L-forms was inhibited by the bacteriocin to the same extent as their parent strains. The staphylococcin was adsorbed to cell walls, cell membranes, and resistant cells. The effect of staphylococcin 1580 appeared to be bactericidal but not bacteriolytic.
Authors:
A M Jetten; G D Vogels
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  112     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1972 Oct 
Date Detail:
Created Date:  1972-12-26     Completed Date:  1972-12-26     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  243-50     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adsorption
Amino Acids / analysis
Bacteria / drug effects
Bacterial Proteins / analysis
Bacteriocins* / analysis,  metabolism,  pharmacology
Carbohydrates / analysis
Cell Membrane / metabolism
Cell Wall / metabolism
Chromatography, Paper
Chromatography, Thin Layer
Chymotrypsin / pharmacology
Drug Resistance, Microbial
Hot Temperature
L Forms / drug effects
Lipids / analysis
Pronase / pharmacology
Species Specificity
Staphylococcus* / analysis,  drug effects
Trypsin / pharmacology
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Bacterial Proteins; 0/Bacteriocins; 0/Carbohydrates; 0/Lipids; EC 3.4.21.1/Chymotrypsin; EC 3.4.21.4/Trypsin; EC 3.4.24.-/Pronase
Comments/Corrections

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