Document Detail


Nature of cholinesterase in the rat retina.
MedLine Citation:
PMID:  8207326     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The occurrence of cholinesterases has been demonstrated in retinas of several mammalian species. Histochemical staining techniques indicate that the acetylcholinesterases (AChE) are present in amacrine cells and their neighboring bipolar cells. However, the nature of retinal cholinesterases and their interactions with specific cholinesterase inhibitors are not known. Therefore, we have studied the inhibition of the rat retinal cholinesterase activity by BW284C51, a selective inhibitor of AChE, and iso-OMPA, a selective inhibitor of butyrylcholinesterase (BChE). Retinas from Zivic-Miller rats were solubilized by sonication in phosphate buffer (0.134 M, pH 7.2) at 4 degrees C for 20 min. The cholinesterase activity in the sonicate was determined by a radiometric method using 14C-acetylcholine (ACh) as substrate (10(-2) M). Excess 14C-ACh was adsorbed by Amberlite CG-120 cation exchange resin. 14C-acetate formed and retained in the aqueous medium was determined by liquid scintillation counting. This study gave the following results: (a) Rat retinal sonicate gave total cholinesterase activity of 3.76 mumol of ACh hydrolyzed/mg protein/15 min; (b) This activity was inhibited by BW284C51 (IC50, 0.115 microM). Iso-OMPA (IC50, 500 microM) did not cause significant inhibition at 0.115 microM. These observations suggest that the rat retinal cholinesterase is predominantly AChE.
Authors:
B V Sastry; S R Kambam; G Singh; J J Franks
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of ocular pharmacology     Volume:  10     ISSN:  8756-3320     ISO Abbreviation:  J Ocul Pharmacol     Publication Date:  1994  
Date Detail:
Created Date:  1994-07-13     Completed Date:  1994-07-13     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8511297     Medline TA:  J Ocul Pharmacol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  195-201     Citation Subset:  IM    
Affiliation:
Department of Anesthesiology, Vanderbilt University Medical Center, Nashville, Tennessee.
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MeSH Terms
Descriptor/Qualifier:
Animals
Benzenaminium, 4,4'-(3-oxo-1,5-pentanediyl)bis(N,N-dimethyl-N-2-propenyl-), Dibromide / pharmacology
Cholinesterase Inhibitors / pharmacology
Cholinesterases / classification,  metabolism*
Organophosphorus Compounds / pharmacology
Rats
Rats, Sprague-Dawley
Retina / drug effects,  enzymology*
Grant Support
ID/Acronym/Agency:
NIDA DA 06207/DA/NIDA NIH HHS
Chemical
Reg. No./Substance:
0/Cholinesterase Inhibitors; 0/Organophosphorus Compounds; 152-16-9/octamethyl pyrophosphoramide; 402-40-4/Benzenaminium, 4,4'-(3-oxo-1,5-pentanediyl)bis(N,N-dimethyl-N-2-propenyl-), Dibromide; EC 3.1.1.8/Cholinesterases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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