Document Detail

Nature and biosynthesis of sialic acids in the starfish Asterias rubens. Identification of sialo-oligomers and detection of S-adenosyl-L-methionine: N-acylneuraminate 8-O-methyltransferase and CMP-N-acetylneuraminate monooxygenase activities.
MedLine Citation:
PMID:  1576206     Owner:  NLM     Status:  MEDLINE    
Mass spectrometric and NMR spectroscopic analyses of bound sialic acids from the starfish Asterias rubens revealed the presence of N-acetylneuraminic acid (4%), N-acetyl-8-O-methylneuraminic acid (12%), N-acetyl-9-O-acetyl-8-O-methylneuraminic acid (less than 1%), N-glycoloylneuraminic acid (19%), N-glycoloyl-8-O-methylneuraminic acid (47%), and N-glycoloyl-9-O-acetyl-8-O-methylneuraminic acid (18%). Analysis of sialo-oligomeric material, obtained after mild acid hydrolysis, demonstrated that N-glycoloyl-8-O-methylneuraminic acid can occur as di- and tri-oligomers, linked through the anomeric center and the N-glycoloyl moiety, Neu5Gc8Me-alpha(2----O5)-Neu5Gc8Me and Neu5Gc8Me-alpha(2----O5)-Neu5Gc8Me-alpha (2----O5)-Neu5Gc8Me. Studies on the biosynthesis of N-acyl-8-O-methylneuraminic acid in A rubens, using the tracer S-adenosyl-L-[methyl-14C]methionine, showed that N-acylneuraminate 8-O-methyltransferase activity was present predominantly in the membrane fraction. CMP-N-acetylneuraminic acid monooxygenase activity was found in the soluble protein fraction, in agreement with investigations on the corresponding vertebrate enzyme.
A A Bergwerff; S H Hulleman; J P Kamerling; J F Vliegenthart; L Shaw; G Reuter; R Schauer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimie     Volume:  74     ISSN:  0300-9084     ISO Abbreviation:  Biochimie     Publication Date:  1992 Jan 
Date Detail:
Created Date:  1992-06-08     Completed Date:  1992-06-08     Revised Date:  2012-05-24    
Medline Journal Info:
Nlm Unique ID:  1264604     Medline TA:  Biochimie     Country:  FRANCE    
Other Details:
Languages:  eng     Pagination:  25-37     Citation Subset:  IM    
Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, The Netherlands.
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MeSH Terms
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Magnetic Resonance Spectroscopy
Methyltransferases / metabolism*
Mixed Function Oxygenases / metabolism*
S-Adenosylmethionine / analysis*
Sialic Acids / biosynthesis*
Starfish / metabolism*
Reg. No./Substance:
0/Sialic Acids; 29908-03-0/S-Adenosylmethionine; EC 1.-/Mixed Function Oxygenases; EC monooxygenase; EC 2.1.1.-/Methyltransferases; EC 2.1.1.-/N-acylneuraminate 8-0-methyltransferase

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