Document Detail

Naturally occurring testis-specific histone H3 antisense transcripts in Drosophila.
MedLine Citation:
PMID:  9364435     Owner:  NLM     Status:  MEDLINE    
While analysing the transcription of the cluster of cell-cycle regulated histone genes in Drosophila hydei, we have found transcripts spanned both histone H3 and H4 genes and were antisense for histone H3. As the two histone genes are in opposite orientation, these transcripts contained the sense strand of the histone H4 gene. Such transcripts were present in both poly(A)+ and poly(A)- RNA fractions. The polyadenylated molecules contained a poly(A) tail at the 3' end of the stem-loop structure, which is characteristic for cell-cycle regulated histone mRNAs. The antisense RNA of histone H3 is synthesized exclusively in testes. By developing an improved protocol of in situ hybridization to Drosophila testis squashes, we could demonstrate that the antisense transcripts are localized in the nuclei of primary spermatocytes. Possible functions of this RNA are discussed.
A Akhmanova; H Kremer; K Miedema; W Hennig
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular reproduction and development     Volume:  48     ISSN:  1040-452X     ISO Abbreviation:  Mol. Reprod. Dev.     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1998-01-27     Completed Date:  1998-01-27     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8903333     Medline TA:  Mol Reprod Dev     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  413-20     Citation Subset:  IM    
Department of Molecular and Developmental Genetics, Faculty of Sciences, Catholic University of Nijmegen, The Netherlands.
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MeSH Terms
Base Sequence
Cell Nucleus / metabolism
DNA Probes / genetics
Drosophila / genetics*,  growth & development
Genes, Insect
Histones / genetics*
In Situ Hybridization
Organ Specificity
Promoter Regions, Genetic
RNA, Antisense / genetics*,  metabolism
Spermatocytes / metabolism
Spermatogenesis / genetics
Testis / growth & development,  metabolism
Transcription, Genetic
Reg. No./Substance:
0/DNA Probes; 0/Histones; 0/RNA, Antisense

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