Document Detail


Natural odor ligands for olfactory receptor neurons of the female mosquito Aedes aegypti: use of gas chromatography-linked single sensillum recordings.
MedLine Citation:
PMID:  18775939     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Female Aedes aegypti are vectors of dengue and yellow fever. Odor volatiles are the predominant cues that drive the host-seeking behavior of Ae. aegypti. Odorant molecules are detected and discriminated by olfactory receptor neurons (ORNs) housed in sensory hairs, sensilla, located on the antennae and maxillary palps. In a previous study, we used odor volatiles that are behaviorally and/or electrophysiologically active for Ae. aegypti and other mosquito species to show that antennal ORNs of female Ae. aegypti are divided into functionally different classes. In the present study, we have, for the first time, conducted gas chromatography-coupled single sensillum recordings (GC-SSR) from antennal trichoid and intermediate sensilla of female Ae. aegypti in order to screen for additional putative host attractants and repellents. We used headspace collections from biologically relevant sources, such as different human body parts (including feet, trunk regions and armpit), as well as a plant species used as a mosquito repellent, Nepeta faassenii. We found that a number of ORN types strongly responded to one or more of the biological extracts. GC-SSR recordings revealed several active components, which were subsequently identified through GC-linked mass spectrometry (GC-MS). Electrophysiologically active volatiles from human skin included heptanal, octanal, nonanal and decanal.
Authors:
Majid Ghaninia; Mattias Larsson; Bill S Hansson; Rickard Ignell
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of experimental biology     Volume:  211     ISSN:  0022-0949     ISO Abbreviation:  J. Exp. Biol.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-09-08     Completed Date:  2008-12-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0243705     Medline TA:  J Exp Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  3020-7     Citation Subset:  IM    
Affiliation:
SLU, Department of Plant Protection Biology, 230 53 Alnarp, Sweden. majid.ghaninia@vv.slu.se
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MeSH Terms
Descriptor/Qualifier:
Adult
Aedes / physiology*
Aldehydes / pharmacology*
Animals
Chromatography, Gas
Female
Humans
Male
Odors*
Olfactory Perception
Olfactory Receptor Neurons / drug effects,  physiology*
Sensitivity and Specificity
Stimulation, Chemical
Volatilization
Chemical
Reg. No./Substance:
0/Aldehydes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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