| Natural killer cell stimulatory factor (NKSF) augments natural killer cell and antibody-dependent tumoricidal response against colon carcinoma cell lines. | |
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MedLine Citation:
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PMID: 1673486 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The therapy of colorectal cancer may be improved by biologic response modifiers that enhance natural killer (NK) cell and antibody-dependent tumoricidal mechanisms. This study examined the effect of a recently discovered cytokine purified from the supernatant of an Ebstein-Barr virus-transformed B-lymphoblastoid cell line (RPMI-8866), natural killer cell stimulatory factor (NKSF), on NK and antibody-dependent cellular cytotoxicity (ADCC) of human colon adenocarcinoma cell lines. Human peripheral blood lymphocytes were cultured for 24 hr in the presence or absence of NKSF (3.6 pM) or interleukin-2 (1 nM). The cultured lymphocytes were analyzed for lytic potential toward chromium-51-labeled colon carcinoma targets SW 1116, 498 LI, and WC 1. ADCC was measured by incubating chromium-51-labeled SW 1116 or WC 1 targets with the monoclonal antibody CO17-1A, an IgG2a antibody reactive with gastrointestinal cancer-associated cell antigen, or control mouse IgG prior to testing NKSF-treated or control PBL effectors in a 6-hr cytotoxicity assay. NKSF significantly enhanced NK cytolysis of colon carcinoma and NK-resistant lymphoma cell lines, and on a molar basis was approximately 300 times more potent than interleukin-2 in generating NK cytotoxicity. Furthermore, NKSF significantly augmented lymphocyte-mediated ADCC against colon carcinoma targets, and the combination of NKSF with the antibody CO17-1A had an additive effect on lymphocyte tumoricidial capacity. Thus, NKSF may have a potential role in the treatment of colon cancer. |
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Authors:
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M D Lieberman; R K Sigal; N N Williams; J M Daly |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of surgical research Volume: 50 ISSN: 0022-4804 ISO Abbreviation: J. Surg. Res. Publication Date: 1991 Apr |
Date Detail:
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Created Date: 1991-05-28 Completed Date: 1991-05-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376340 Medline TA: J Surg Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 410-5 Citation Subset: IM |
Affiliation:
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Department of Surgical Research, University of Pennsylvania, School of Medicine, Philadelphia 19104. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibody-Dependent Cell Cytotoxicity / drug effects* Carcinoma / immunology*, pathology Colonic Neoplasms / immunology*, pathology HLA Antigens / analysis Humans Immunity, Cellular / drug effects Interleukin-12 Interleukins / pharmacology* Killer Cells, Natural / immunology* Mice Mice, Nude Neoplasm Transplantation Receptors, Fc / metabolism Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/HLA Antigens; 0/Interleukins; 0/Receptors, Fc; 187348-17-0/Interleukin-12 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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