| Natural killer cell-mediated antitumor reactivity of rhesus monkeys. | |
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MedLine Citation:
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PMID: 3878943 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have analyzed natural killer (NK) cell-mediated antitumor activity or peripheral blood mononuclear cells of rhesus monkeys. All monkeys displayed significant NK cell cytolytic activity against the human tumor cell lines K-562, Daudi and CEM in a short-term (3 h) 51Cr-release assay. Similar to NK cells described in other species, the cytotoxic cells of monkeys were relatively nonadherent to nylon wool columns, exhibited low density after separation on discontinuous Percoll density gradients, and displayed large granular lymphocyte (LGL) morphology. Analysis of the mechanism of NK cell cytotoxicity of rhesus monkeys demonstrated that on the average, 7.1% (range: 3.1-13.2%) of lymphocytes bound to K-562 tumor, and that approximately 14.8% (range: 7.9-26.3%) of these tumor-binding cells (TBC) were cytolytically active. Examination of TBC on cytocentrifuge slides indicated that the majority of binders displayed LGL morphology. The cytotoxic reaction mediated by monkey NK cells exhibited Michaelis-Menten kinetics pattern; the maximum rate of lysis (Vmax) of K-562 was found to be 1-2 X 10(4) following 3 h of incubation. Using similar culture conditions, the recycling capacity of NK cells of this species was estimated at 2-6 times. Finally, it was observed that the NK cell activity of most monkeys could be potentiated following in vitro exposure to the biological response modifier, interleukin-2. |
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Authors:
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C A Savary; E Lotzová; K N Gray; J H Jardine |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Natural immunity and cell growth regulation Volume: 4 ISSN: 0254-7600 ISO Abbreviation: Nat. Immun. Cell Growth Regul. Publication Date: 1985 |
Date Detail:
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Created Date: 1986-03-11 Completed Date: 1986-03-11 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8407979 Medline TA: Nat Immun Cell Growth Regul Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 328-39 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle / drug effects Child Cytotoxicity, Immunologic* / drug effects Humans Interleukin-2 / pharmacology Killer Cells, Natural / cytology, drug effects, immunology*, physiology, transplantation Leukemia, Myeloid / immunology* Lymphocyte Activation / drug effects Lymphocytes / classification Macaca / immunology* Macaca mulatta / immunology* |
| Grant Support | |
ID/Acronym/Agency:
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CA 12542/CA/NCI NIH HHS; CA 31394/CA/NCI NIH HHS; CA 39632/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Interleukin-2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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