Document Detail


Natural killer T cells are required for the development of a superantigen-driven T helper type 2 immune response in mice.
MedLine Citation:
PMID:  16162272     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We show, here, that one single injection or weekly injections of staphylococcal enterotoxin B (SEB), starting in 1-day-old newborn mice, induced a powerful immune response with a T helper type 2 (Th2) pattern, as judged by the isotype and cytokine profile, with the production of large amounts of SEB-specific immunoglobulin G1 (IgG1), detectable levels of SEB-specific IgE and increased production of interleukin-4 by spleen cells. These protocols also induced an increase in the levels of total IgE in the serum. Memory of SEB was transferred to secondary recipients by using total spleen cells from primed animals. The secondary humoral response in transferred mice was diminished if spleen cells from SEB-treated mice were previously depleted of CD3+ or Vbeta8+ T cells or NK1.1+ cells. In vivo depletion of NK1.1+ cells in adult mice resulted in a marked reduction in the SEB-specific antibody response in both the primary and secondary immune responses. Additionally, purified NK1.1+ T cells were able to perform SEB-specific helper B-cell actions in vitro and in vivo. These results suggest that NK1.1+ T cells are required for the full development of humoral immunological memory, whilst making neonatal tolerance to SEB unachievable.
Authors:
Auro Nomizo; Edilberto Postol; Raquel de Alencar; Fabíola Cardillo; José Mengel
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology     Volume:  116     ISSN:  0019-2805     ISO Abbreviation:  Immunology     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-09-15     Completed Date:  2005-11-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  England    
Other Details:
Languages:  eng     Pagination:  233-44     Citation Subset:  IM    
Affiliation:
Department of Clinical Analysis, Toxicology and Bromatology, Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adoptive Transfer
Animals
Animals, Newborn
Antibodies, Bacterial / biosynthesis
Antigens / analysis
Antigens, Ly
Antigens, Surface
Cells, Cultured
Enterotoxins / immunology
Enzyme-Linked Immunosorbent Assay / methods
Immune Tolerance
Immunoglobulin E / biosynthesis
Immunoglobulin G / biosynthesis
Immunologic Memory
Interleukin-4 / biosynthesis
Killer Cells, Natural / immunology*
Lectins, C-Type
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
NK Cell Lectin-Like Receptor Subfamily B
Proteins / analysis
Spleen / immunology,  transplantation
Superantigens / immunology*
T-Lymphocyte Subsets / immunology
T-Lymphocytes, Helper-Inducer / immunology
Th2 Cells / immunology*
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Antigens; 0/Antigens, Ly; 0/Antigens, Surface; 0/Enterotoxins; 0/Immunoglobulin G; 0/Klrb1c protein, mouse; 0/Lectins, C-Type; 0/NK Cell Lectin-Like Receptor Subfamily B; 0/Proteins; 0/Superantigens; 207137-56-2/Interleukin-4; 37341-29-0/Immunoglobulin E; 39424-53-8/enterotoxin B, staphylococcal
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Cholesterol depletion inhibits src family kinase-dependent calcium mobilization and apoptosis induce...
Next Document:  Qualitatively distinct patterns of cytokines are released by human dendritic cells in response to di...