Document Detail

Natural human tumor necrosis factor beta (lymphotoxin). Variable O-glycosylation at Thr7, proteolytic processing, and allelic variation.
MedLine Citation:
PMID:  1451807     Owner:  NLM     Status:  MEDLINE    
Natural human tumor necrosis factor beta (TNF-beta) purified from supernatants of a human B-lymphoblastoid cell line was found to be heterogeneous in molecular mass, with seven components resolved by gel electrophoresis. All components are N-glycosylated at Asn62; N-glycosylation does not contribute to heterogeneity. In addition, part of the molecules are O-glycosylated at Thr7; O-glycosylation is heterogeneous due to variable decoration with neuraminic acid. The four lower molecular mass forms are derived from the full-length protein by trypsin-like proteolytic cleavage in the N-proximal region; these clipped molecules lack O-linked carbohydrates. Two allelic variants differing in amino acid position 26 (threonine/asparagine) were identified.
C G Voigt; I Maurer-Fogy; G R Adolf
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEBS letters     Volume:  314     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1992-12-31     Completed Date:  1992-12-31     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  85-8     Citation Subset:  IM    
Ernst Boehringer-Institut für Arzneimittelforschung, Bender & Co. GmbH, Vienna, Austria.
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MeSH Terms
Amino Acid Sequence
Asparagine / metabolism
B-Lymphocytes / chemistry*
Genetic Variation
Lymphotoxin-alpha / genetics*,  isolation & purification,  metabolism*
Molecular Sequence Data
Neuraminic Acids / analysis
Protein Processing, Post-Translational*
Threonine / metabolism
Reg. No./Substance:
0/Lymphotoxin-alpha; 0/Neuraminic Acids; 7006-34-0/Asparagine; 72-19-5/Threonine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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