Document Detail


Natural human tumor necrosis factor beta (lymphotoxin). Variable O-glycosylation at Thr7, proteolytic processing, and allelic variation.
MedLine Citation:
PMID:  1451807     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Natural human tumor necrosis factor beta (TNF-beta) purified from supernatants of a human B-lymphoblastoid cell line was found to be heterogeneous in molecular mass, with seven components resolved by gel electrophoresis. All components are N-glycosylated at Asn62; N-glycosylation does not contribute to heterogeneity. In addition, part of the molecules are O-glycosylated at Thr7; O-glycosylation is heterogeneous due to variable decoration with neuraminic acid. The four lower molecular mass forms are derived from the full-length protein by trypsin-like proteolytic cleavage in the N-proximal region; these clipped molecules lack O-linked carbohydrates. Two allelic variants differing in amino acid position 26 (threonine/asparagine) were identified.
Authors:
C G Voigt; I Maurer-Fogy; G R Adolf
Related Documents :
647477 - Biosynthesis of chloramphenicol in streptomyces sp. 3022a. properties of an aminotransf...
8437 - Purification and properties of an endo-cellulase of avicelase type from irpex lacteus (...
6614917 - Multiple forms of rat dentin phosphoproteins.
8312877 - Controlled-release preparation of indomethacin using calcium alginate gel.
23770037 - Schistosoma mansoni: assessment of effects of oleic acid, cercarial age and water tempe...
76687 - Radioimmunochemical measurement of bone marrow prostatic acid phosphatase.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEBS letters     Volume:  314     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1992-12-31     Completed Date:  1992-12-31     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  85-8     Citation Subset:  IM    
Affiliation:
Ernst Boehringer-Institut für Arzneimittelforschung, Bender & Co. GmbH, Vienna, Austria.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Alleles
Amino Acid Sequence
Asparagine / metabolism
B-Lymphocytes / chemistry*
Genetic Variation
Glycosylation
Humans
Lymphotoxin-alpha / genetics*,  isolation & purification,  metabolism*
Molecular Sequence Data
Neuraminic Acids / analysis
Protein Processing, Post-Translational*
Threonine / metabolism
Chemical
Reg. No./Substance:
0/Lymphotoxin-alpha; 0/Neuraminic Acids; 7006-34-0/Asparagine; 72-19-5/Threonine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Complex formation between glutamyl-tRNA synthetase and glutamyl-tRNA reductase during the tRNA-depen...
Next Document:  'All-or-none' mechanism of the molten globule unfolding.