| Natural human tumor necrosis factor beta (lymphotoxin). Variable O-glycosylation at Thr7, proteolytic processing, and allelic variation. | |
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MedLine Citation:
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PMID: 1451807 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Natural human tumor necrosis factor beta (TNF-beta) purified from supernatants of a human B-lymphoblastoid cell line was found to be heterogeneous in molecular mass, with seven components resolved by gel electrophoresis. All components are N-glycosylated at Asn62; N-glycosylation does not contribute to heterogeneity. In addition, part of the molecules are O-glycosylated at Thr7; O-glycosylation is heterogeneous due to variable decoration with neuraminic acid. The four lower molecular mass forms are derived from the full-length protein by trypsin-like proteolytic cleavage in the N-proximal region; these clipped molecules lack O-linked carbohydrates. Two allelic variants differing in amino acid position 26 (threonine/asparagine) were identified. |
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Authors:
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C G Voigt; I Maurer-Fogy; G R Adolf |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: FEBS letters Volume: 314 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1992 Dec |
Date Detail:
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Created Date: 1992-12-31 Completed Date: 1992-12-31 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 85-8 Citation Subset: IM |
Affiliation:
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Ernst Boehringer-Institut für Arzneimittelforschung, Bender & Co. GmbH, Vienna, Austria. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alleles Amino Acid Sequence Asparagine / metabolism B-Lymphocytes / chemistry* Genetic Variation Glycosylation Humans Lymphotoxin-alpha / genetics*, isolation & purification, metabolism* Molecular Sequence Data Neuraminic Acids / analysis Protein Processing, Post-Translational* Threonine / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Lymphotoxin-alpha; 0/Neuraminic Acids; 7006-34-0/Asparagine; 72-19-5/Threonine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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