Document Detail


Natural human antibodies retrieved by phage display libraries from healthy donors: polyreactivity and recognition of human immunodeficiency virus type 1gp120 epitopes.
MedLine Citation:
PMID:  10447936     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have characterized the human natural antibody repertoire that contains antibodies recognizing the human immunodeficiency virus type 1 (HIV-1) gp120. A panel of monovalent antigen-binding fragments (Fab) selected from IgM and IgG isotype libraries generated from peripheral blood mononuclear cells (PBMC) of a healthy, HIV-1 noninfected individual was analysed, reflecting that only IgM, but not IgG, Fab were able to recognize HIV-1 gp120. The IgM Fab antibodies were not restricted to any particular heavy chain variable region (VH) germ line gene. However, the recognition of gp120 is associated to polyreactive antibodies and all display low-affinity interaction. This correlates with the absence of any maturation process as somatic mutation or isotype switch as the nucleotide sequence analysis of the variable regions reveals they are expressed near to germline configuration. In addition, none of the antibodies showed any neutralizing activity on HIV-1-infected lymphocytes, reflecting that the natural anti-gp120 repertoire is not sufficient to neutralize HIV infection.
Authors:
M Llorente; S Sánchez-Palomino; S Mañes; P Lucas; L Kremer; I M De Alborán; J L Torán; J Alcamí; G Del Real; C Martínez-A
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Scandinavian journal of immunology     Volume:  50     ISSN:  0300-9475     ISO Abbreviation:  Scand. J. Immunol.     Publication Date:  1999 Sep 
Date Detail:
Created Date:  1999-09-23     Completed Date:  1999-09-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0323767     Medline TA:  Scand J Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  270-9     Citation Subset:  IM; X    
Affiliation:
Department of Immunology and Oncology, Centro Nacional de Biotecnología, CSIC/UAM, Campus de Cantoblanco, E-28049, Madrid, Spain.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / biosynthesis,  genetics,  immunology*
Bacteriophages
Base Sequence
Blood Donors
Cross Reactions
Epitopes, B-Lymphocyte / immunology*
Genes, Immunoglobulin
HIV Antibodies / biosynthesis,  genetics,  immunology*
HIV Envelope Protein gp120 / immunology*
HIV-1 / immunology*
Humans
Immunoglobulin Fab Fragments / immunology
Immunoglobulin Heavy Chains / genetics
Immunoglobulin Variable Region / genetics
Molecular Sequence Data
Peptide Library
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Epitopes, B-Lymphocyte; 0/HIV Antibodies; 0/HIV Envelope Protein gp120; 0/Immunoglobulin Fab Fragments; 0/Immunoglobulin Heavy Chains; 0/Immunoglobulin Variable Region; 0/Peptide Library

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