Document Detail


Natural history of paroxysmal nocturnal hemoglobinuria clones in patients presenting as aplastic anemia.
MedLine Citation:
PMID:  21447004     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE:  To investigate the natural history of paroxysmal nocturnal hemoglobinuria (PNH) clones in patients with acquired aplastic anemia (AA).
PATIENTS AND METHODS:  Twenty-seven patients with AA and a detectable PNH clone were monitored for a median of 5.7 years (range 1.5-11.5 years). Twenty-two patients received high-dose cyclophosphamide (HiCy) therapy. The erythrocyte and granulocyte PNH clone sizes were measured using flow cytometry and analyzed via CellQuest software. PE-conjugated anti-glycophorin A, anti-CD15, FITC-conjugated anti-CD59, and FLAER staining were used to define glycosylphosphatidylinositol-AP-deficient cells.
RESULTS: We found a linear relationship between PNH clone size and the development of intravascular hemolysis, assessed by lactate dehydrogenase (LDH) values (Pearson correlation coefficient = 0.80, P < 0.001 for erythrocyte PNH clones; and Pearson correlation coefficient = 0.73, P < 0.0001 for granulocyte PNH clones). An erythrocyte PNH size of 3-5% and granulocyte PNH size of 23% were the thresholds to predict hemolysis as measured by an elevated LDH (receiver operating characteristic analyses with AUC = 0.96 for erythrocyte PNH clone sizes and AUC = 0.88 for granulocyte PNH clone sizes). Patients with small (≤15%) initial PNH clone sizes were less likely to develop an elevated LDH (mean ± SD: 236.9 ± 109.9 vs. 423.1 ± 248.8; P = 0.02). Over time, the PNH clone sizes remained stable in 25.9% of patients; 48.1% experienced a rise in the PNH clone size; and 25.9% experienced a decrease.
CONCLUSION: The risk of developing clinically significant PNH after HiCy therapy appears to be low in AA patients with PNH clones, especially for those with small initial PNH clones and for those who respond to HiCy therapy.
Authors:
Jeffrey J Pu; Galina Mukhina; Hao Wang; William J Savage; Robert A Brodsky
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  European journal of haematology     Volume:  87     ISSN:  1600-0609     ISO Abbreviation:  Eur. J. Haematol.     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-06-22     Completed Date:  2011-08-24     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  8703985     Medline TA:  Eur J Haematol     Country:  England    
Other Details:
Languages:  eng     Pagination:  37-45     Citation Subset:  IM    
Copyright Information:
© 2011 John Wiley & Sons A/S.
Affiliation:
Division of Hematology, Department of Medicine, School of Medicine, Johns Hopkins University, Baltimore, MD, USA.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Anemia, Aplastic / blood,  complications*,  drug therapy
Child
Clone Cells / pathology
Cyclophosphamide / therapeutic use
Erythrocytes / pathology
Female
Granulocytes / pathology
Hemoglobinuria, Paroxysmal / blood,  drug therapy,  etiology*
Humans
Immunosuppressive Agents / therapeutic use
L-Lactate Dehydrogenase / blood
Male
Middle Aged
Young Adult
Grant Support
ID/Acronym/Agency:
P01CA70970/CA/NCI NIH HHS; T32 HL007525-28/HL/NHLBI NIH HHS; T32HL007525/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Immunosuppressive Agents; 50-18-0/Cyclophosphamide; EC 1.1.1.27/L-Lactate Dehydrogenase
Comments/Corrections

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