Document Detail


Natural history of fetal cell microchimerism during and following murine pregnancy.
MedLine Citation:
PMID:  15949558     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In humans, fetal cells enter the maternal circulation during all pregnancies and can persist for decades. Human studies, however, are often limited by the number of subjects and the availability of healthy and diseased tissues for analysis. We sought to develop a murine model to establish the natural history of fetal cell microchimerism in various maternal tissues during and after healthy pregnancies resulting from congenic and allogenic matings. We bred C57BL/6J and DBA/2J virgin female mice to C57BL/6J males transgenic for the enhanced green fluorescent protein (GFP), which shows autosomal dominant inheritance with complete penetrance and is under the control of a ubiquitous chicken beta-actin promoter and a cytomegalovirus enhancer. During pregnancy and at different times after delivery, female mice were sacrificed. Tissues were collected and the presence of the gfp transgene and GFP+ cells was assessed by real-time quantitative PCR and by immunofluorescence. During pregnancy, microchimerism was detected in all tissues from mice carrying GFP+ fetuses. Fetal cells were often mononuclear. The frequency of fetal cells in the lungs was significantly higher compared to other tissues. The level of microchimerism was also significantly higher in congenic compared to allogenic matings. After delivery, the frequency of fetal cells decreased and fetal cells were undetectable at 2 and 3 weeks after the first delivery. However, some mice that had three gestations had detectable fetal cells 3 weeks after their last delivery. Using sensitive methods of detection, we demonstrate that fetal cell microchimerism occurs during all murine pregnancies. We describe a useful model for the study of the consequences of this phenomenon.
Authors:
Kiarash Khosrotehrani; Kirby L Johnson; Sarah Guégan; Helene Stroh; Diana W Bianchi
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of reproductive immunology     Volume:  66     ISSN:  0165-0378     ISO Abbreviation:  J. Reprod. Immunol.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-06-13     Completed Date:  2005-10-18     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8001906     Medline TA:  J Reprod Immunol     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  1-12     Citation Subset:  IM    
Affiliation:
Division of Genetics, Department of Obstetrics and Gynecology, Tufts-New England Medical Center, 750 Washington Street, P.O. Box 394, Boston, MA 02111, USA.
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MeSH Terms
Descriptor/Qualifier:
Actins / genetics
Animals
Chimerism / embryology*
Female
Fetus / chemistry,  cytology*
Green Fluorescent Proteins / analysis,  genetics
Lung / cytology,  embryology
Male
Mice
Models, Animal*
Penetrance
Pregnancy*
Promoter Regions, Genetic
Transgenes
Grant Support
ID/Acronym/Agency:
P30 DK34928/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Actins; 0/enhanced green fluorescent protein; 147336-22-9/Green Fluorescent Proteins

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