Document Detail


Natural cycle IVF and oocyte in-vitro maturation in polycystic ovary syndrome: a collaborative prospective study.
MedLine Citation:
PMID:  19146766     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In-vitro maturation (IVM) was performed in 350 cycles for 262 unstimulated patients diagnosed with polycystic ovary syndrome who were primed with human chorionic gonadotrophin (HCG) before oocyte retrieval. In order to improve nuclear and cytoplasmic maturation, growth hormone was added to the maturation medium. Oocytes were recovered in 94.8% of the cycles, with a mean number of nine cumulus-oocyte complexes retrieved. Within 28 h, 62% of the oocytes reached the metaphase II (MII) stage, and 17.6% were MII after a further 20 h in culture. An ongoing pregnancy rate of 15.2% was obtained, but with a high miscarriage rate, 28% of the total with a positive betaHCG test assessed after embryo transfer. Cytogenetic and DNA fragmentation analysis of the embryos was not fundamentally different from what is classically observed in routine IVF. This observation implies that the results are not necessarily due to compromised oocyte quality after IVM, and that endometrial receptivity should also be considered, especially in IVM cycles where the follicular phase is dramatically shortened.
Authors:
M Benkhalifa; A Demirol; Y Ménézo; E Balashova; A K Abduljalil; S Abbas; I Giakoumakis; T Gurgan
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Publication Detail:
Type:  Clinical Trial; Journal Article; Multicenter Study    
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  18     ISSN:  1472-6491     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-01-16     Completed Date:  2009-02-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  England    
Other Details:
Languages:  eng     Pagination:  29-36     Citation Subset:  IM    
Affiliation:
ATL R&D, Reproductive Biology and Genetics Laboratory, La Verriere, France.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aneuploidy
Cell Culture Techniques
Cell Differentiation / genetics
Cells, Cultured
Cooperative Behavior
DNA Fragmentation
Embryo Transfer
Female
Fertilization in Vitro / methods*
Humans
Menstrual Cycle / physiology*
Metaphase / physiology
Oocytes / physiology
Oogenesis / genetics,  physiology*
Polycystic Ovary Syndrome / physiopathology*
Pregnancy
Treatment Outcome

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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