| Natural course of iron delocalization and lipid peroxidation during the first eight hours following a 15-minute cardiac arrest in dogs. | |
MedLine Citation:
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PMID: 3662177 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lipid peroxidation is thought to be a major contributing factor in neurological injury following cardiac arrest. Because iron availability is a prerequisite for lipid peroxidation, this experiment was designed to examine the natural time course of iron release, lipid peroxidation, and cerebral polyunsaturated fatty acid content following a 15-minute cardiac arrest in dogs. Large mongrel dogs were anesthetized with ketamine and halothane and divided into three groups of five each. In two groups, cardiac arrest was induced with KCl. After 15 minutes of cardiac arrest, the dogs were resuscitated by five minutes of internal cardiac massage, epinephrine, bicarbonate, and internal defibrillation. All ten dogs were resuscitated and supported by a standard intensive care protocol until tissue harvest. A 3-g portion of parietal cerebral cortex was obtained from the nonischemic dogs (n = 5), or at two hours (n = 5), or eight hours (n = 5) after resuscitation. Total tissue iron was measured by an atomic emission spectrometer; low molecular weight species (LMWS) iron by the o-phenanthroline test on an ultrafiltered sample; and lipid peroxidation by both the thiobarbituric acid test (TBARS) and determination of the tissue content of lipid double bonds, calculated by first fractionating the lipids by gas-liquid chromatography and then measuring the double bonds in each fraction by spectrometry and summing the results. Univariate ANOVA demonstrated all variables except total tissue iron to have significance at P less than .02. At two hours of reperfusion, LMWS iron and TBARS were significantly elevated above nonischemic control levels.(ABSTRACT TRUNCATED AT 250 WORDS) |
Authors:
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G S Krause; N R Nayini; B C White; T J Hoenher; A M Garritano; B J O'Neil; S D Aust |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Annals of emergency medicine Volume: 16 ISSN: 0196-0644 ISO Abbreviation: Ann Emerg Med Publication Date: 1987 Nov |
Date Detail:
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Created Date: 1987-11-18 Completed Date: 1987-11-18 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8002646 Medline TA: Ann Emerg Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1200-5 Citation Subset: AIM; IM |
Affiliation:
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Department of Surgery, Wayne State University School of Medicine, Detroit, Michigan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / metabolism* Brain Ischemia / metabolism Cell Survival Dogs Heart Arrest / metabolism* Iron / metabolism* Membrane Lipids / metabolism* Molecular Weight Oxidation-Reduction Phospholipids / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Membrane Lipids; 0/Phospholipids; 7439-89-6/Iron |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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