| Natural and azido fatty acids inhibit phosphate transport and activate fatty acid anion uniport mediated by the mitochondrial phosphate carrier. | |
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MedLine Citation:
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PMID: 11085992 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The electroneutral P(i) uptake via the phosphate carrier (PIC) in rat liver and heart mitochondria is inhibited by fatty acids (FAs), by 12-(4-azido-2-nitrophenylamino)dodecanoic acid (AzDA) and heptylbenzoic acid ( approximately 1 microm doses) and by lauric, palmitic, or 12-azidododecanoic acids ( approximately 0.1 mm doses). In turn, reconstituted E. coli-expressed yeast PIC mediated anionic FA uniport with a similar pattern leading to FA cycling and H(+) uniport. The kinetics of P(i)/P(i) exchange on recombinant PIC in the presence of AzDA better corresponded to a competitive inhibition mechanism. Methanephosphonate was identified as a new PIC substrate. Decanephosphonate, butanephosphonate, 4-nitrophenylphosphate, and other P(i) analogs were not translocated and did not inhibit P(i) transport. However, methylenediphosphonate and iminodi(methylenephosphonate) inhibited both electroneutral P(i) uptake and FA cycling via PIC. AzDA analog 16-(4-azido-2-nitrophenylamino)-[(3)H(4)]-hexadecanoic acid ((3)H-AzHA) bound upon photoactivation to several mitochondrial proteins, including the 30- and 34-kDa bands. The latter was ascribed to PIC due to its specific elution pattern on Blue Sepharose and Affi-Gel. (3)H-AzHA photolabeling of recombinant PIC was prevented by methanephosphonate and diphosphonates and after premodification with 4-azido-2-nitrophenylphosphate. Hence, the demonstrated PIC interaction with monovalent long-chain FA anions, but with divalent phosphonates of short chain only, indicates a pattern distinct from that valid for the mitochondrial uncoupling protein-1. |
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Authors:
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H Engstová; M Zácková; M Růzicka; A Meinhardt; J Hanus; R Krämer; P Jezek |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2000-11-20 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 276 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2001 Feb |
Date Detail:
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Created Date: 2001-05-23 Completed Date: 2001-06-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 4683-91 Citation Subset: IM |
Affiliation:
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Institute of Physiology, Department of Membrane Transport Biophysics, Academy of Sciences of the Czech Republic, Prague CZ 14220, Czech Republic. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Affinity Labels
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pharmacology Animals Biological Transport, Active Carrier Proteins / antagonists & inhibitors*, metabolism* Diphosphonates / pharmacology Dose-Response Relationship, Drug Fatty Acids / metabolism, pharmacology* Ion Transport Kinetics Lauric Acids / pharmacology* Mitochondria / drug effects, metabolism Palmitic Acids / pharmacology Phosphate-Binding Proteins Phosphates / metabolism* Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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0/12-(4-azido-2-nitrophenylamino)dodecanoic acid; 0/16-(4-azido-2-nitrophenylamino)hexadecanoic acid; 0/Affinity Labels; 0/Carrier Proteins; 0/Diphosphonates; 0/Fatty Acids; 0/Lauric Acids; 0/Palmitic Acids; 0/Phosphate-Binding Proteins; 0/Phosphates |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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