Document Detail

Natural antibodies against bone marrow cells of a concordant xenogeneic species.
MedLine Citation:
PMID:  2051029     Owner:  NLM     Status:  MEDLINE    
Hyperacute rejection does not occur when vascularized organs are transplanted between rat and mouse, and this species combination is considered to be concordant. Since hyperacute rejection is believed to reflect the presence of pre-existing antibodies (usually of the IgM class), and lymphocytotoxic antibodies against rat cells have not been detected in normal mouse sera, it has previously been concluded that mouse anti-rat natural antibody (NAb) does not exist. However, studies have not been reported in which rat bone marrow cells (BMC) were used as targets for evaluation of normal mouse sera. Because previous work from our and other laboratories has shown that bone marrow chimerism in the rat into mouse species combination can be achieved only by transplanting large numbers of rat BMC, we have evaluated normal mouse sera for the presence of NAb against rat BMC that might explain these in vivo results. Fisher 344 rat BMC and spleen cells were incubated with serum from nonimmunized mice, then stained with fluoresceinated rat anti-mouse subclass-specific secondary reagents and analyzed using flow cytometry. NAb of the IgM and IgG3 classes were found that bound strongly to rat BMC but showed weak or absent binding to spleen cells. A low level of IgG2b binding was observed to both BMC and spleen cells. Cytotoxic activity was detected against rat BMC but not against spleen cells. The environment in which the animals were maintained played a significant role in determining the level of cytotoxic NAb in normal mouse sera. Our results are consistent with the possibility that bone marrow-specific NAb play a role in resisting engraftment of BMC across this species barrier.
I Aksentijevich; D H Sachs; M Sykes
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  147     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1991 Jul 
Date Detail:
Created Date:  1991-07-23     Completed Date:  1991-07-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  79-85     Citation Subset:  AIM; IM    
Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
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MeSH Terms
Antibodies / immunology*
Antibody-Dependent Cell Cytotoxicity
Bone Marrow / immunology*
Bone Marrow Cells
Bone Marrow Transplantation / immunology*
Cytotoxicity, Immunologic
Mice, Inbred Strains
Rats, Inbred Strains
Species Specificity
Spleen / immunology
Transplantation, Heterologous
Reg. No./Substance:

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