Document Detail


Natural abundance Carbon-13 nuclear magnetic resonance spectroscopy of liver and adipose tissue of the living rat.
MedLine Citation:
PMID:  6639939     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have employed the topical magnetic resonance (TMR) technique to obtain natural abundance 13C nuclear magnetic resonance (NMR) spectra from liver and adipose tissue in the living rat. Experiments were performed in a TMR magnet (20-cm diameter) with a two-turn radio-frequency coil ("surface" coil) combined with a focused static magnetic field. The in vivo spectra that were obtained at 20.2 MHz have been assigned by comparison with those from excised organs obtained in a conventional spectrometer operating at 90.5 MHz. Signals in the TMR spectra corresponding to carbons of the carbohydrates, glucose and glycogen, and of the lipids, triglycerides and phospholipids, have been resolved in vivo and assigned. The effects of chronic modification of dietary fat and carbohydrate on the in vivo spectra have been investigated. The levels of carbohydrates and of saturated and unsaturated fats in the liver as measured by 13C TMR reflect the relative amounts of these compounds in the long-term diet.
Authors:
P Canioni; J R Alger; R G Shulman
Related Documents :
8159259 - Analysis of magnetic resonance spectra by mole percent: comparison to absolute units.
17637209 - Magnetic resonance spectroscopy in parkinson's disease and parkinsonian syndromes.
10643589 - Molecular aspects of magnetic resonance imaging and spectroscopy.
21800159 - Cross-sectional imaging obtained immediately following radiofrequency atrial fibrillati...
7843719 - 31p magnetic resonance spectroscopy of the liver: correlation with standardized serum, ...
9402199 - Magnetization transfer imaging in vivo of the rat brain at 4.7 t: interpretation using ...
19864699 - Diffusion and near-equilibrium distribution of mri and ct contrast agents in articular ...
9798239 - Sensitivity of various radiographic methods for detection of oral cancellous bone lesions.
17850289 - Impact of heart rhythm status on registration accuracy of the left atrium for catheter ...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemistry     Volume:  22     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  1983 Oct 
Date Detail:
Created Date:  1984-01-27     Completed Date:  1984-01-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4974-80     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adipose Tissue / analysis*
Animals
Carbon Isotopes
Diet
Fatty Acids / analysis
Glucose / analysis
Lipids / analysis
Liver / analysis*
Liver Glycogen / analysis
Magnetic Resonance Spectroscopy / methods
Male
Rats
Rats, Inbred Strains
Grant Support
ID/Acronym/Agency:
AM 21721/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 0/Fatty Acids; 0/Lipids; 0/Liver Glycogen; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Reinvestigation of the shape and state of hydration of the skeletal myosin subfragment 1 monomer in ...
Next Document:  Functions of the 5'-phosphoryl group of pyridoxal 5'-phosphate in phosphorylase: a study using pyrid...