Document Detail


Natural Leishmania sp. reservoirs and phlebotomine sandfly food source identification in Ibitipoca State Park, Minas Gerais, Brazil.
MedLine Citation:
PMID:  22666858     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Leishmania spp are distributed throughout the world and different species are associated with varying degrees of disease severity. However, leishmaniasis is thought to be confined to areas of the world where its insect vectors, sandflies, are present. Phlebotomine sandflies obtain blood meals from a variety of wild and domestic animals and sometimes from humans. These vectors transmit Leishmania spp, the aetiological agent of leishmaniasis. Identification of sandfly blood meals has generally been performed using serological methods, although a few studies have used molecular procedures in artificially fed insects. In this study, cytochrome b gene (cytB) polymerase chain reaction (PCR) was performed in DNA samples isolated from 38 engorged Psychodopygus lloydi and the expected 359 bp fragment was identified from all of the samples. The amplified product was digested using restriction enzymes and analysed for restriction fragment length polymorphisms (RFLPs). We identified food sources for 23 females; 34.8% yielded a primate-specific banding profile and 26.1% and 39.1% showed banding patterns specific to birds or mixed restriction profiles (rodent/marsupial, human/bird, rodent/marsupial/human), respectively. The food sources of 15 flies could not be identified. Two female P. lloydi were determined to be infected by Leishmania using internal transcribed spacer 1 and heat shock protein 70 kDa PCR-RFLP. The two female sandflies, both of which fed on rodents/marsupials, were further characterised as infected with Leishmania (Viannia) braziliensis. These results constitute an important step towards applying methodologies based on cytB amplification as a tool for identifying the food sources of female sandflies.
Authors:
Patrícia Flávia Quaresma; Gustavo Mayr de Lima Carvalho; Mariana Campos das Neves Farah Ramos; José Dilermando Andrade Filho
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Memórias do Instituto Oswaldo Cruz     Volume:  107     ISSN:  1678-8060     ISO Abbreviation:  Mem. Inst. Oswaldo Cruz     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-06-05     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502619     Medline TA:  Mem Inst Oswaldo Cruz     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  480-5     Citation Subset:  IM    
Affiliation:
Laboratório de Leishmanioses, Instituto René Rachou, Fiocruz, Belo Horizonte, Minas Gerais, Brasil, 30190-002.
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