| Natural IgM and innate immune collectin SP-D bind to late apoptotic cells and enhance their clearance by alveolar macrophages in vivo. | |
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MedLine Citation:
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PMID: 21035192 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Innate immune collectin surfactant protein D (SP-D) and natural immunoglobulin M (IgM) are two soluble proteins. These opsonic proteins are good candidates for enhancing late apoptotic cell clearance. However, effects of these proteins on late apoptotic cell clearance in the lungs are not clearly established. We have recently shown that SP-D can bind several immunoglobulin isotypes, including IgM. Here we hypothesized that IgM and SP-D bind to late apoptotic cells and enhance their clearance from the lungs. We show that IgM and SP-D bind to late apoptotic secondary necrotic cells, and that IgM and SP-D either co-localize to the same regions or to different regions of late apoptotic Jurkat T cells. Mouse alveolar macrophages internalized late apoptotic cells, in vivo. We induced lung inflammation in mice using LPS and show that airway IgM and SP-D levels and the clearance of late apoptotic cells by alveolar macrophages increases under these conditions. We then coated late apoptotic cells with IgM, SP-D, or both and instilled them into the mouse airways. We found that alveolar macrophages internalize IgM- and SP-D-coated late apoptotic cells more effectively than uncoated late apoptotic cells, in vivo. None of these conditions cause inflammation in the naïve lungs. Therefore, these data suggest that both IgM and SP-D effectively opsonize late apoptotic cells and directly enhance their clearance by alveolar macrophages in the lungs. |
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Authors:
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Michael L Litvack; Pascal Djiadeu; Sri Dushyaanthan Sri Renganathan; Sarah Sy; Martin Post; Nades Palaniyar |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-29 |
Journal Detail:
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Title: Molecular immunology Volume: 48 ISSN: 1872-9142 ISO Abbreviation: Mol. Immunol. Publication Date: 2010 Nov-Dec |
Date Detail:
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Created Date: 2010-11-29 Completed Date: 2011-01-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7905289 Medline TA: Mol Immunol Country: England |
Other Details:
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Languages: eng Pagination: 37-47 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Lung Innate Immunity Research Laboratory, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / immunology* Blotting, Western Cell Separation Enzyme-Linked Immunosorbent Assay Flow Cytometry Immunity, Innate / immunology* Immunoglobulin M / immunology* Inflammation / immunology Macrophages, Alveolar / immunology* Mice Pulmonary Surfactant-Associated Protein D / immunology* |
| Grant Support | |
ID/Acronym/Agency:
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MOP-134761//Canadian Institutes of Health Research; MOP-84312//Canadian Institutes of Health Research |
| Chemical | |
Reg. No./Substance:
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0/Immunoglobulin M; 0/Pulmonary Surfactant-Associated Protein D |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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