Document Detail

Natriuretic peptides inhibit apoptosis and prolong the survival of serum-deprived PC12 cells.
MedLine Citation:
PMID:  11209918     Owner:  NLM     Status:  MEDLINE    
Atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) were investigated to determine effects on apoptotic DNA fragmentation and survival in serum-deprived PC12 cells. Both peptides caused prolonged cGMP (but not cAMP) elevations lasting for > or = 6 h. The cGMP elevations were 10-, 50- and 68-fold for ANP and 26-, 100- and 148-fold for BNP at 1, 10 and 100 nM, respectively. BNP caused dose-dependent increases in cell survival rates during 3 days of serum deprivation. BNP (1 nM) increased 24 h survival rate from 36% to 67%. ANP (1 nM), BNP (1 nM) and 8-bromo-cGMP (0.1 mM) inhibited by 74.8%, 46.7% and 86.8%, respectively, the apoptotic DNA fragmentation in serum-deprived PC12 cells, measured by our recently developed quantitative technique using capillary electrophoresis with laser-induced fluorescence detector (CE-LIF). The data suggest prolonged cGMP elevations caused by ANP or BNP inhibit apoptotic DNA fragmentation and prolong the survival of serum-deprived PC12 cells.
R R Fiscus; A W Tu; S B Chew
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroreport     Volume:  12     ISSN:  0959-4965     ISO Abbreviation:  Neuroreport     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-02-07     Completed Date:  2001-03-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9100935     Medline TA:  Neuroreport     Country:  England    
Other Details:
Languages:  eng     Pagination:  185-9     Citation Subset:  IM    
Department of Physiology, Faculty of Medicine, The Epithelial Cell Biology Research Centre, The Chinese University of Hong Kong, Shatin, NT.
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MeSH Terms
Apoptosis / drug effects*
Atrial Natriuretic Factor / pharmacology*
Cell Survival / drug effects
Culture Media, Serum-Free / pharmacology*
Cyclic GMP / analogs & derivatives*,  metabolism,  pharmacology
DNA Fragmentation
Dose-Response Relationship, Drug
Electrophoresis, Capillary
Natriuretic Peptide, Brain / pharmacology*
Neurons / cytology,  drug effects*,  metabolism
Neuroprotective Agents / pharmacology
PC12 Cells
Reg. No./Substance:
0/Culture Media, Serum-Free; 0/Neuroprotective Agents; 114471-18-0/Natriuretic Peptide, Brain; 31356-94-2/8-bromocyclic GMP; 7665-99-8/Cyclic GMP; 85637-73-6/Atrial Natriuretic Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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