| Nascent HDL formation in hepatocytes and role of ABCA1, ABCG1 and SR-BI. | |
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MedLine Citation:
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PMID: 22190590 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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To study the mechanisms of hepatic HDL formation, we investigated the roles of ABCA1, ABCG1 and SR-BI in nascent HDL formation in primary hepatocytes isolated from mice deficient in ABCA1, ABCG1, or SR-BI and from wild type (WT) mice. Under basal conditions, in WT hepatocytes, cholesterol efflux to exogenous apoA-I was accompanied by conversion of apoA-I to HDL-sized particles. LXR activation by T0901317 markedly enhanced the formation of larger HDL-sized particles as well as cellular cholesterol efflux to apoA-I. Glyburide treatment completely abolished the formation of 7.4 nm diameter and greater particles, but led to the formation of novel 7.2 nm-sized particles. However, cells lacking ABCA1 failed to form such particles. ABCG1- deficient cells showed similar capacity to efflux cholesterol to apoA-I and to form nascent HDL particles compared to WT cells. Cholesterol efflux to apoA-I and nascent HDL formation were slightly but significantly enhanced in SR-BI- deficient cells compared with WT cells under basal but not LXR activated conditions. As in WT but not in ABCA1- deficient hepatocytes, 7.2 nm-sized particles generated by glyburide treatment were also detected in ABCG1-deficient and SR-BI-deficient hepatocytes. Our data indicate that hepatic nascent HDL formation is highly dependent on ABCA1, but not on ABCG1 or SR-BI. |
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Authors:
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Ailing Ji; Joanne M Wroblewski; Lei Cai; Maria C de Beer; Nancy R Webb; Deneys R van der Westhuyzen |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-12-20 |
Journal Detail:
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Title: Journal of lipid research Volume: - ISSN: 0022-2275 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-22 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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University of Kentucky, United States. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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