Document Detail


Nasal glandular secretory response to cholinergic stimulation in humans and guinea pigs.
MedLine Citation:
PMID:  1778947     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A guinea pig model of nasal secretory responses was developed to assess the contributions of vascular permeability and glandular secretion responsible for the production of cholinergically stimulated nasal secretions. The nasal secretory responses to provocation with saline, methacholine, and atropine on the ipsilateral (challenged) side and contralateral (reflex) side were analyzed by measurement of total protein (Lowry method), guinea pig albumin (enzyme-linked immunosorbent assay), 125I-labeled bovine serum albumin after intravenous injection, and alkaline phosphatase enzyme activity in nasal fluid. Alkaline phosphatase was found to be localized to submucosal glands by zymography. Topical methacholine challenge increased the secretion of total protein, alkaline phosphatase activity, and albumin on the ipsilateral challenged side, whereas the percentage of total protein represented by albumin was not increased. This response was totally prevented by atropine pretreatment. Serial provocation with methacholine resulted in progressively reduced amounts of both the total protein and alkaline phosphatase in secretions. The observation that repeated challenges produced progressively smaller responses was also examined employing human nasal provocation. Repeating methacholine (25 mg) challenges four times at 10-min intervals in six human volunteers revealed that the initial challenge produced the largest response as reflected in total protein, albumin, lysozyme, lactoferrin, immunoglobulin (Ig) G, IgA, and secretory IgA secretion. When the constituents in secretions were analyzed in relationship to the total protein, the two vascular proteins, IgG and albumin, demonstrated the greatest decrements with repeated methacholine challenges. The glandular proteins, lactoferrin, lysozyme, and secretory IgA, either remained constant or increased in their relative proportion to total protein. Thus, cholinergic stimulation causes glandular secretion from both the guinea pig and human nasal mucosa.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
A Z Gawin; B E Emery; J N Baraniuk; M A Kaliner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  71     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  1991 Dec 
Date Detail:
Created Date:  1992-03-12     Completed Date:  1992-03-12     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2460-8     Citation Subset:  IM    
Affiliation:
Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Albumins / secretion
Alkaline Phosphatase / metabolism
Animals
Atropine / pharmacology*
Guinea Pigs
Humans
Immunoglobulins / metabolism
Male
Methacholine Chloride / pharmacology*
Middle Aged
Nasal Mucosa / drug effects*,  enzymology,  secretion*
Proteins / secretion
Chemical
Reg. No./Substance:
0/Albumins; 0/Immunoglobulins; 0/Proteins; 51-55-8/Atropine; 62-51-1/Methacholine Chloride; EC 3.1.3.1/Alkaline Phosphatase

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