Document Detail


Nasal absorption and local tissue reaction of insulin nanocomplexes of trimethyl chitosan derivatives in rats.
MedLine Citation:
PMID:  20609059     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: The objective of this work was to explore the potential and safety of trimethyl chitosan (TMC) and PEGylated TMC for improved absorption of insulin after nasal administration.
METHODS: The nasal absorption of insulin nanocomplexes of TMC or PEGylated TMC was evaluated in anaesthetized rats. Concomitantly, the histopathological effects of these nanocomplexes on rat nasal mucosa were studied using a perfusion fixation technique.
KEY FINDINGS: All insulin nanocomplexes containing TMC or PEGylated TMC showed a 34-47% reduction in the blood glucose concentration, when the insulin absorption through the rat nasal mucosa was measured indirectly. In addition, the relative pharmacodynamic bioavailability (F(dyn)) of the formulations was found to be dependent upon the charge ratio of insulin and polymer, regardless of polymer structure. The F(dyn) apparently decreased with increasing charge ratio of insulin : polymer. Although acute alterations in nasal morphology by the formulations were affected by the charge ratio of insulin and polymer, the formulation of insulin/PEGylated TMC nanocomplexes was shown to be less toxic to the nasal epithelial membrane than insulin/TMC nanocomplexes.
CONCLUSIONS: PEGylated TMC nanocomplexes were a suitable absorption enhancer for nasal delivery of insulin.
Authors:
Anchalee Jintapattanakit; Penchom Peungvicha; Achariya Sailasuta; Thomas Kissel; Varaporn Buraphacheep Junyaprasert
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of pharmacy and pharmacology     Volume:  62     ISSN:  2042-7158     ISO Abbreviation:  J. Pharm. Pharmacol.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-07-08     Completed Date:  2011-01-31     Revised Date:  2011-09-13    
Medline Journal Info:
Nlm Unique ID:  0376363     Medline TA:  J Pharm Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  583-91     Citation Subset:  IM    
Affiliation:
Department of Pharmacy, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
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MeSH Terms
Descriptor/Qualifier:
Absorption
Administration, Intranasal
Animals
Biological Availability
Biophysical Processes
Blood Glucose / metabolism*
Chemistry, Pharmaceutical / methods
Chitosan / administration & dosage,  adverse effects,  pharmacokinetics*
Drug Carriers / administration & dosage,  pharmacokinetics,  toxicity
Hypoglycemic Agents / administration & dosage,  pharmacokinetics*,  pharmacology
Insulin / administration & dosage,  pharmacokinetics*,  pharmacology
Male
Nanoparticles
Nasal Mucosa / drug effects*,  pathology
Polyethylene Glycols / administration & dosage,  adverse effects,  pharmacokinetics*
Polymers / chemistry
Rats
Rats, Wistar
Technology, Pharmaceutical / methods*
Chemical
Reg. No./Substance:
0/Blood Glucose; 0/Drug Carriers; 0/Hypoglycemic Agents; 0/Polyethylene Glycols; 0/Polymers; 11061-68-0/Insulin; 9012-76-4/Chitosan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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