Document Detail

Narrow Host-Range Bacteriophages that Infect Rhizobium etli associate with Distinct Genomic Types.
MedLine Citation:
PMID:  24185856     Owner:  NLM     Status:  Publisher    
In this work, we isolated and characterized 14 bacteriophages that infect Rhizobium etli. They were obtained from rhizosphere soil of bean plants of agriculture lands in México using the enrichment method. The host range of these phages was narrow but variable within a collection of 48 R. etli strains. We obtained the complete genome sequence of nine phages. Four phages were resistant to several restriction enzymes and in vivo cloning, probably due to nucleotide modifications. The genome size of the sequenced phages varied from 43 Kb to 115 Kb with a median size of ∼45 to 50 Kb. A large proportion of open reading frames of these phage genomes consisted of hypothetical and orphan genes (65-70%). The remainder encoded proteins needed for phage morphogenesis, DNA synthesis and processing, among others, and a minor percentage represented genes of bacterial origin. We classified these phages into four genomic types based on their genomic similarity, gene content, and host range. Since there are no reports of similar sequences, we propose that these bacteriophages correspond to novel species.
Rosa Isela Santamaría; Patricia Bustos; Omar Sepúlveda-Robles; Luis Lozano; César Rodríguez; José Luis Fernández; Soledad Juárez; Luis Kameyama; Gabriel Guarneros; Guillermo Dávila; Víctor González
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-11-1
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  -     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2013 Nov 
Date Detail:
Created Date:  2013-11-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Programa de Genómica Evolutiva, Centro de Ciencias Genómicas, UNAM. Av. Universidad N/C Col. Chamilpa CP 62310. Apdo. Postal 565-A. Cuernavaca, Morelos, México.
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