Document Detail


Naphthoquinone components from Alkanna tinctoria (L.) Tausch show significant antiproliferative effects on human colorectal cancer cells.
MedLine Citation:
PMID:  22473633     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Our research to seek active compounds against human colorectal cancer from the root of Alkanna tinctoria (L.) Tausch led to the isolation of two naphthoquinones, alkannin (1) and angelylalkannin (2). The antiproliferative effects of the two compounds on human colon cancer cells HCT-116 and SW-480 were determined by the 3,4-(5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium salt (MTS) method. Cell cycle profile and cell apoptosis were determined using flow cytometry. Both of the two compounds showed significant inhibitory effects on the cancer cells. For alkannin (1) and angelylalkannin (2), the median inhibitory concentration (IC₅₀) values were 2.38 and 4.76 µM for HCT-116 cells, while for SW-480 cells they were 4.53 and 7.03 µM, respectively. The potential antiproliferative mechanisms were also explored. At concentrations between 1-10 µM, both compounds arrested the cell cycle at the G1 phase and induced cell apoptosis.
Authors:
Nguyen Huu Tung; Guang-Jian Du; Chong-Zhi Wang; Chun-Su Yuan; Yukihiro Shoyama
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-04-04
Journal Detail:
Title:  Phytotherapy research : PTR     Volume:  27     ISSN:  1099-1573     ISO Abbreviation:  Phytother Res     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-08     Completed Date:  2013-04-26     Revised Date:  2014-03-19    
Medline Journal Info:
Nlm Unique ID:  8904486     Medline TA:  Phytother Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  66-70     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 John Wiley & Sons, Ltd.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antineoplastic Agents, Phytogenic / pharmacology*
Apoptosis / drug effects
Boraginaceae / chemistry*
Cell Cycle / drug effects
Cell Proliferation / drug effects*
Colorectal Neoplasms
HCT116 Cells
Humans
Naphthoquinones / pharmacology*
Plant Extracts / pharmacology*
Plant Roots / chemistry
Rats
Grant Support
ID/Acronym/Agency:
AT004418/AT/NCCAM NIH HHS; AT005362/AT/NCCAM NIH HHS; K01 AT005362/AT/NCCAM NIH HHS; P01 AT004418/AT/NCCAM NIH HHS
Chemical
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/Naphthoquinones; 0/Plant Extracts; 0/angelylalkannin; 075CRZ9995/alkannin
Comments/Corrections

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