Document Detail


Nanotopology of cell adhesion upon Variable-Angle Total Internal Reflection Fluorescence Microscopy (VA-TIRFM).
MedLine Citation:
PMID:  23052337     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Surface topology, e.g. of cells growing on a substrate, is determined with nanometer precision by Variable-Angle Total Internal Reflection Fluorescence Microscopy (VA-TIRFM). Cells are cultivated on transparent slides and incubated with a fluorescent marker homogeneously distributed in their plasma membrane. Illumination occurs by a parallel laser beam under variable angles of total internal reflection (TIR) with different penetration depths of the evanescent electromagnetic field. Recording of fluorescence images upon irradiation at about 10 different angles permits to calculate cell-substrate distances with a precision of a few nanometers. Differences of adhesion between various cell lines, e.g. cancer cells and less malignant cells, are thus determined. In addition, possible changes of cell adhesion upon chemical or photodynamic treatment can be examined. In comparison with other methods of super-resolution microscopy light exposure is kept very small, and no damage of living cells is expected to occur.
Authors:
Michael Wagner; Petra Weber; Harald Baumann; Herbert Schneckenburger
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Video-Audio Media     Date:  2012-10-02
Journal Detail:
Title:  Journal of visualized experiments : JoVE     Volume:  -     ISSN:  1940-087X     ISO Abbreviation:  J Vis Exp     Publication Date:  2012  
Date Detail:
Created Date:  2012-10-11     Completed Date:  2012-12-12     Revised Date:  2014-10-14    
Medline Journal Info:
Nlm Unique ID:  101313252     Medline TA:  J Vis Exp     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e4133     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Adhesion / physiology*
Cell Line, Tumor
Cell Membrane / chemistry,  metabolism
Fluorescent Dyes / chemistry,  metabolism
Glioblastoma / genetics,  metabolism,  pathology
Humans
Image Processing, Computer-Assisted
Microscopy, Fluorescence / instrumentation,  methods*
Tumor Suppressor Protein p53 / biosynthesis,  genetics
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/TP53 protein, human; 0/Tumor Suppressor Protein p53
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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