Document Detail


Nanostructured Superhydrophobic Substrates Trigger the Development of 3D Neuronal Networks.
MedLine Citation:
PMID:  23027505     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The generation of 3D networks of primary neurons is a big challenge in neuroscience. Here, a novel method is presented for a 3D neuronal culture on superhydrophobic (SH) substrates. How nano-patterned SH devices stimulate neurons to build 3D networks is investigated. Scanning electron microscopy and confocal imaging show that soon after plating neurites adhere to the nanopatterned pillar sidewalls and they are subsequently pulled between pillars in a suspended position. These neurons display an enhanced survival rate compared to standard cultures and develop mature networks with physiological excitability. These findings underline the importance of using nanostructured SH surfaces for directing 3D neuronal growth, as well as for the design of biomaterials for neuronal regeneration.
Authors:
Tania Limongi; Fabrizia Cesca; Francesco Gentile; Roberto Marotta; Roberta Ruffilli; Andrea Barberis; Marco Dal Maschio; Enrica Maria Petrini; Stefania Santoriello; Fabio Benfenati; Enzo Di Fabrizio
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-2
Journal Detail:
Title:  Small (Weinheim an der Bergstrasse, Germany)     Volume:  -     ISSN:  1613-6829     ISO Abbreviation:  Small     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101235338     Medline TA:  Small     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
Department of Nanostructures, Istituto Italiano di Tecnologia, Via Morego 30 - 16163 Genova, Italy; Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Via Morego 30 - 16163 Genova, Italy.
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