Document Detail


Nanostimulation: manipulation of single neuron activity by juxtacellular current injection.
MedLine Citation:
PMID:  19955285     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the mammalian brain, many thousands of single-neuron recording studies have been performed but less than 10 single-cell stimulation studies. This paucity of single-cell stimulation data reflects a lack of easily applicable single-cell stimulation techniques. We provide a detailed description of the procedures involved in nanostimulation, a single-cell stimulation method derived from the juxtacellular labeling technique. Nanostimulation is easy to apply and can be directed to a wide variety of identifiable neurons in anesthetized and awake animals. We describe the recording approach and the parameters of the electric configuration underlying nanostimulation. We use glass pipettes with a DC resistance of 4-7 Mohms. Obtaining the juxtacellular configuration requires a close contact between pipette tip and neuron and is associated with a several-fold increase in resistance to values > or = 20 Mohms. The recorded action potential (AP) amplitude grows to > or = 2 mV, and neurons can be activated with currents in the nanoampere range--hence the term nanostimulation. While exact AP timing has not been achieved, AP frequency and AP number can be parametrically controlled. We demonstrate that nanostimulation can also be used to selectively inhibit sensory responses in identifiable neurons. Nanostimulation is biophysically similar to electroporation, and based on this assumption, we argue that nanostimulation operates on membranes in the micrometer area directly below the pipette tip, where membrane pores are induced by high transmembrane voltage. There is strong evidence to suggest that nanostimulation selectively activates single neurons and that the evoked effects are cell-specific. Nanostimulation therefore holds great potential for elucidating how single neurons contribute to behavior.
Authors:
Arthur R Houweling; Guy Doron; Birgit C Voigt; Lucas J Herfst; Michael Brecht
Related Documents :
18347345 - Chemical transmission between dopaminergic neuron pairs.
11815645 - Patch-clamp analysis of voltage-activated and chemically activated currents in the vome...
19917565 - Homeostasis of intrinsic excitability in hippocampal neurones: dynamics and mechanism o...
12061505 - Ion regulation in the brain: implications for pathophysiology.
18325545 - Effects of age of acquisition on brain activation during chinese character recognition.
18653205 - Jararhagin, a snake venom metalloprotease-disintegrin, activates the rac1 gtpase and st...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-12-02
Journal Detail:
Title:  Journal of neurophysiology     Volume:  103     ISSN:  1522-1598     ISO Abbreviation:  J. Neurophysiol.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-17     Completed Date:  2010-06-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375404     Medline TA:  J Neurophysiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1696-704     Citation Subset:  IM    
Affiliation:
Bernstein Center for Computational Neuroscience, Humboldt University of Berlin, Berlin, Germany. a.houweling@erasmusmc.nl
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Action Potentials / physiology
Anesthesia
Animals
Biophysics
Electric Stimulation / methods*
Electroporation
Head Movements / physiology
Iontophoresis
Micromanipulation / methods*
Motor Cortex / cytology,  physiology
Neurons / physiology*
Rats
Rats, Wistar

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Adaptive changes in anticipatory postural adjustments with novel and familiar postural supports.
Next Document:  Quantitative estimation of calcium dynamics from ratiometric measurements: A direct, non-ratioing, m...