Document Detail


Nanoshells for surface-enhanced Raman spectroscopy in eukaryotic cells: cellular response and sensor development.
MedLine Citation:
PMID:  19807067     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The application of gold nanoshells (NS) as a surface-enhanced Raman (SER) platform for intracellular sensing in NIH-3T3 fibroblast cells was studied by using a near-infrared Raman system. To show the feasibility of using these 151 +/- 5 nm sized solution-stable nanoparticles inside living cells, we investigated the uptake, cellular response, and the health of the cell population. We show that NS are taken up voluntarily and can be found in the cytosol by transmission electron microscopy (TEM), which also provides detailed information about location and immediate surrounding of the NS. The internalization into cells has been found to be independent of active cellular mechanisms, such as endocytosis, and can be suggested to be of passive nature. Uptake of NS into cells can be controlled, and cells show no increase in necrosis or apoptosis as a result; we show that NS-based intracytosolic SER spectra can be measured on biological samples using short acquisition times and low laser powers. We demonstrate its application using 4-mercaptobenzoic acid (4-MBA)-functionalized nanoshells as a pH sensor.
Authors:
Michael A Ochsenk??hn; Phillip R T Jess; Helene Stoquert; Kishan Dholakia; Colin J Campbell
Related Documents :
19128047 - Live hela cells preconcentrate and differentiate inorganic arsenic species.
15847587 - Photothermal responses of individual cells.
11889087 - Force and velocity of mycoplasma mobile gliding.
20394437 - Dendrimer internalization and intracellular trafficking in living cells.
6204097 - Abnormal expression of vimentin intermediate filaments in human lymphoid cell lines wit...
7725327 - Clostridium sordellii cytotoxin induces phosphorylation of an 80,000 mol. wt protein in...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  ACS nano     Volume:  3     ISSN:  1936-086X     ISO Abbreviation:  ACS Nano     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-11-25     Completed Date:  2010-02-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101313589     Medline TA:  ACS Nano     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3613-21     Citation Subset:  IM    
Affiliation:
School of Chemistry, University of Edinburgh, Edinburgh, U.K.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects
Biological Markers / metabolism
Biological Transport
Cell Survival / drug effects
Cytosol / drug effects,  metabolism
Eukaryotic Cells / cytology*,  drug effects*,  metabolism,  ultrastructure
Gold / chemistry*,  metabolism,  pharmacology*
Hydrogen-Ion Concentration
Mice
Microscopy, Electron, Transmission
NIH 3T3 Cells
Nanoshells / chemistry*
Necrosis / metabolism
Particle Size
Silicon Dioxide / chemistry
Spectrum Analysis, Raman
Surface Plasmon Resonance
Surface Properties
Chemical
Reg. No./Substance:
0/Biological Markers; 7440-57-5/Gold; 7631-86-9/Silicon Dioxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Localization of Na(+)-K(+) ATPases in Quasi-Native Cell Membranes.
Next Document:  Temperature-controlled syntheses of substituted 1,2,4-triazolelead(II) complexes: active lone pair a...