Document Detail


Nanoscale structure of type I collagen fibrils: quantitative measurement of D-spacing.
MedLine Citation:
PMID:  23027700     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This article details a quantitative method to measure the D-periodic spacing of type I collagen fibrils using atomic force microscopy coupled with analysis using a two-dimensional fast fourier transform approach. Instrument calibration, data sampling and data analysis are discussed and comparisons of the data to the complementary methods of electron microscopy and X-ray scattering are made. Examples of the application of this new approach to the analysis of type I collagen morphology in disease models of estrogen depletion and osteogenesis imperfecta (OI) are provided. We demonstrate that it is the D-spacing distribution, not the D-spacing mean, that showed statistically significant differences in estrogen depletion associated with early stage osteoporosis and OI. The ability to quantitatively characterize nanoscale morphological features of type I collagen fibrils will provide important structural information regarding type I collagen in many research areas, including tissue aging and disease, tissue engineering, and gene knockout studies. Furthermore, we also envision potential clinical applications including evaluation of tissue collagen integrity under the impact of diseases or drug treatments.
Authors:
Blake Erickson; Ming Fang; Joseph M Wallace; Bradford G Orr; Clifford M Les; Mark M Banaszak Holl
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-10-24
Journal Detail:
Title:  Biotechnology journal     Volume:  8     ISSN:  1860-7314     ISO Abbreviation:  Biotechnol J     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-02     Completed Date:  2013-09-12     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  101265833     Medline TA:  Biotechnol J     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  117-26     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Collagen Type I / chemistry,  ultrastructure*
Female
Fourier Analysis
Microscopy, Atomic Force / methods
Nanotechnology / methods
Ovariectomy
Protein Conformation
Sheep
Skin / chemistry
Grant Support
ID/Acronym/Agency:
1F32DE018840-01A1/DE/NIDCR NIH HHS; AR50562/AR/NIAMS NIH HHS; F32 DE018840/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Collagen Type I
Comments/Corrections

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