| Nanoscale Structure of Type I Collagen Fibrils: Quantitative Measurement of D-spacing. | |
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MedLine Citation:
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PMID: 23027700 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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This paper details a quantitative method to measure the D-periodic spacing of Type I collagen fibrils using Atomic Force Microscopy coupled with analysis using a 2D Fast Fourier Transform approach. Instrument calibration, data sampling and data analysis are all discussed and comparisons of the data to the complementary methods of electron microscopy and X-ray scattering are made. Examples of the application of this new approach to the analysis of Type I collagen morphology in disease models of estrogen depletion and Osteogenesis Imperfecta are provided. We demonstrate that it is the D-spacing distribution, not the D-spacing mean, that showed statistically significant differences in estrogen depletion associated with early stage Osteoporosis and Osteogenesis Imperfecta. The ability to quantitatively characterize nanoscale morphological features of Type I collagen fibrils will provide important structural information regarding Type I collagen in many research areas, including tissue aging and disease, tissue engineering, and gene knock out studies. Furthermore, we also envision potential clinical applications including evaluation of tissue collagen integrity under the impact of diseases or drug treatments. |
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Authors:
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Blake Erickson; Ming Fang; Joseph M Wallace; Bradford G Orr; Clifford M Les; Mark M Banaszak Holl |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-10-2 |
Journal Detail:
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Title: Biotechnology journal Volume: - ISSN: 1860-7314 ISO Abbreviation: Biotechnol J Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-10-2 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101265833 Medline TA: Biotechnol J Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Affiliation:
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The University of Michigan Program in Biophysics; Michigan Nanotechnology Institute for Medicine and Biological Sciences. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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